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OJVRTM

 

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume 29 (3):120-126, 2025.

Effect of antioxidants, peroxidation, chilling and cryopreservation on bovine semen.

 

El-Sisy GA, El-Nattat WS, El-Sheshtawy RI.

 

Department of Animal Reproduction and artificial insemination, National Research Centre, Dokki, Giza, Egypt

 

ABSTRACT

 

El-Sisy GA, El-Nattat WS, El-Sheshtawy RI, Effect of antioxidants, peroxidation, chilling and cryopreservation on bovine semen, Onl J Vet Res, 29 (3):120-126, 2025. Buffalo sperm quality, antioxidant enzymes (SOD and GSH-Px), total antioxidant capacity and lipid peroxidation (MDA) during chilling and cryopreservation were evaluated in 5 Bulls. Sperm motility declined (P < 0.05) from 87% before processing to 83% at dilution, 68 at cooling and 37% after freeze-thawing, membrane integrity from 91% to 84, 73 and 52%, acrosomes from 98% to 96, 77and 50% and live sperm from 97% to 91, 79 and 61%, respectively. SOD in semen declined (P < 0.05) 28% at cooling an 60% at thawing, GSH decreased 33% and 51% and SOD + GSH, 56 and 54%, respectively, whereas MDA increased (P < 0.05) 28% at cooling and 56% by thawing. Our findings suggest declines in antioxidants during cryopreservation may reduce integrity of semen.

 

KEY-WORDS: buffalo – semen – cryopreservation - antioxidants – lipid peroxidation.

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