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OJVRTM
Online Journal of Veterinary
Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 29 (3):120-126, 2025.
Effect of antioxidants, peroxidation, chilling and cryopreservation on
bovine semen.
El-Sisy
GA, El-Nattat WS, El-Sheshtawy
RI.
Department of
Animal Reproduction and artificial insemination, National Research Centre, Dokki, Giza, Egypt
ABSTRACT
El-Sisy GA, El-Nattat WS, El-Sheshtawy RI, Effect of antioxidants, peroxidation, chilling
and cryopreservation on bovine semen, Onl J Vet Res, 29 (3):120-126, 2025. Buffalo sperm quality, antioxidant enzymes (SOD and GSH-Px), total antioxidant capacity and lipid peroxidation
(MDA) during chilling and cryopreservation were evaluated in 5 Bulls. Sperm
motility declined (P < 0.05) from 87% before processing to 83% at dilution,
68 at cooling and 37% after freeze-thawing, membrane integrity from 91% to 84,
73 and 52%, acrosomes from 98% to 96, 77and 50% and live sperm from 97% to 91,
79 and 61%, respectively. SOD in semen declined (P < 0.05) 28% at cooling an 60% at thawing, GSH decreased 33% and 51% and SOD + GSH, 56
and 54%, respectively, whereas MDA increased (P < 0.05) 28% at cooling and
56% by thawing. Our findings suggest declines in antioxidants during cryopreservation
may reduce integrity of semen.
KEY-WORDS: buffalo –
semen – cryopreservation - antioxidants – lipid peroxidation.
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