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Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X


 Volume 25 (6):440-448, 2021.

Effect of C-type natriuretic peptide in diluent for cooling of bull semen.


Mohammedali J Ghafil1, Nazih W Zaid1, Najlaa S Ibrahim1, Faris F Ibrahim2, Kitam J. Yahya2


1Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, 2Ministry of Agriculture Department of Artificial Insemination center-Baghdad, Iraq.




Ghafil M, Zaid N, Ibrahim N, Ibrahim F, Yahya K., Effect of C-type natriuretic peptide diluent for cooling of bull semen, Onl J Vet Res., 25 (6):440-448, 2021.We report mean + se sperm motility (%) and velocity (s) with or without low 10-7 or high concentrations of 10-6 mol/L C-type natriuretic peptide (CNP) over 72 hrs cooling. Controls consisted of standard diluent without CNP. We used 10 semen samples with at least 60%5% motility. Our results show that compared with controls, sperm motility in diluents with CPN was significantly different (P<0.05). For example in controls, sperm motility was ~60% at 0 hrs declining ~36% at 6 hrs and ~96% at 72 hrs whereas with low CPN levels, only declined ~36% at 12 hrs and with high CPN levels only ~50% at 24 hrs and ~85 and ~77%, respectively by 72 hrs. Concerning sperm velocity, we found significant (P < 0.05) increases in those cooled with CNP. For example in controls, velocity was ~11 seconds (s) at 0 hrs declining 15% at 6h and ~42% at 72 hrs whereas with low CPN, increased 63 to 29% over 12h and returned to normal velocity by 72h. In high CPN, these values were even higher with velocity increasing 31 to 80% over 24 h.


Keyword: Natriuretic peptide, diluent, cooling, bull sperm, motility, velocity.