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OJVRTM
Online Journal of Veterinary Research©
Volume 11(2):55-61, 2007 Editors note: Extensively redacted 2018.
Effect of chilling and cryopreservation on
buffalo semen quality, antioxidants and peroxidation.
El-Sisy GA, El-Nattat WS, El-Sheshtawy RI.
Department
of Animal Reproduction and artificial insemination, National Research Centre, Dokki, Giza, Egypt.
ABSTRACT
El-Sisy GA, El-Nattat WS, El-Sheshtawy RI, Effect of chilling and cryopreservation on buffalo semen quality, antioxidants and peroxidation. Onl J Vet Res., 11(2):55-61, 2007. We describe effect of chilling and cryopreservation on sperm quality, antioxidant enzymes (SOD and GSH-Px), total antioxidant capacity and lipid peroxidation from 5 buffalo bulls. Sperm motility declined (P < 0.05) 87% to 68% by cooling and 37% by freeze-thawing, live sperm from 97% to 79.4% and 61%, membrane integrity from 90.8% to 72.8% and 51.2%, and normal acrosomes from 97.8% to 77 and 50% with same parameters. Sperm malonyldehyde (MDA) increased (P < 0.05) from 9.02 ± 1.81 to 12.49 ± 2.19 nmol/l after chilling and 20.69 ± 2.44 nmol/ml after cryopreservation. SOD decreased (P < 0.05) from 5.59 ± 0.04 to 3.98 ± 0.17 after chilling and 2.21 ± 0.08 U/ml after cryopreservation and GSH-Px from 21.68 ± 2.89 to 14.54 ± 2.12 and 10.53 ± 1.51 U/ml with same parameters. Total antioxidants decreased (P < 0.05) from 1.96 ± 0.33 to 0.86 ± 0.06 and 0.90 ± 0.18 mM/L. The results suggest that buffalo spermatozoa is affected by oxidative stress during chilling and cryopreservation due to a decrease in antioxidant enzyme activity.
KEY-WORDS: buffalo – semen – cryopreservation - antioxidants – lipid
peroxidation.