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Online Journal of Veterinary Research©
Volume 23 (8):752-762, 2019.
Effect of ferrous ascorbate on in vitro capacitation of crossbred bull spermatozoa.
Amrit K Bansal° and Gurmail S Bilaspuri *
Department of Animal Breeding & Genetics, Guru Angad Dev Veterinary & Animal Sciences University, Ludhiana-141004, Punjab, India.
Bansal AK, Bilaspuri GS., Effect of ferrous ascorbate on in vitro capacitation of crossbred bull spermatozoa, Onl J Vet Res., 23 (8):752-762, 2019. We induced mild lipid peroxidation (LPO) in crossbred cattle bull spermatozoa with ferrous ascorbate (FeAA – 150 µM FeSO4 : 750 µM ascorbic acid) to evaluate its effect on sperm capacitation and acrosome reaction. Fresh semen samples of crossbred cattle bulls (HHS, Holstein-Friesian x Sahiwal : FC, Friesian Crosses : 1F and 4F, 1st and 4th generation interbreed) were suspended in TALP medium (pH 7.4). The suspension was divided into controls of TALP + spermatozoa) and test ferrous ascorbate (FeAA -150 µM FeSO4 : 750 µM ascorbic acid) + spermatozoa] incubated 6hrs at 37°C and assessed for morphology, % hyperactivity, % acrosome reaction and change in LPO level during capacitation. Morphology of spermatozoa was not affected by FeAA with normal capacitation and acrosome reaction. However, FeAA reduced sperm movement and increased LPO. Results suggested that ascorbate increased reactive oxygen species (ROS)/LPO/oxidative stress without affecting other sperm functions.
Keywords: FeAA, sperm capacitation, acrosome reaction, LPO, % hyperactivity.