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OJVRTM
Online Journal of Veterinary Research©
(Including Medical
and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 28 (12): 822-826, 2024.
Effect of bovine-fetal serum albumin and tissue
culture on in vitro maturation of oocytes.
Yosef Deneke, Prem Singh Yadav, Rajib Deb and Trilok Nanda.
Department
of Animal Biotechnolgy CCS,
HAU, Central Institute for Research on Buffaloes, Hisar,
Division of Animal Biotechnology, Indian Veterinary Research Institute Izatnagar, UttarPradesh-243122,India.
ABSTRACT
Deneke
Y, Yadav PS, Deb R, Nanda T., Effect of bovine-fetal serum albumin and
tissue culture on in vitro maturation
of oocytes, Onl J Vet Res., 28 (12): 822-826, 2024. We report effect of bovine (BSA) and fetal
(FBS) serum albumin and tissue culture 199 for in vitro maturation of buffalo
oocytes, Oocytes were aspirated from abattoir ovarian follicles
2-8mm diameter followed by maturation in TCM-199 supplemented with hCG, PMSG and containing either
0.4% BSA or 10% FCS. Based on cumulus expansion maturation rate, we found higher
viability for oocytes in FBS of ~89 compare to those matured in BSA of ~74%
Key Words: Buffalo oocytes,
in vitro, maturation, bovine, fetal, serum.
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