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OJVRTM
Online
Journal of Veterinary Research©
Volume 23 (10):977-988,
2019.
Quantitative
analysis of toll like receptor 9 mRNA expression by different tissues of
buffalo calves (Bubalus bubalis)
A. Manuja, M. Dhingra,
S. Kumar, S. Sarkar and B. Kumar.
Central Institute for
Research on Buffaloes, Sirsa road, Hisar-125001, Haryana, India
ABSTRACT
Manuja A, Dhingra M, Kumar S, Sarkar S, Kumar B.,
Quantitative analysis of toll like receptor 9 mRNA expression by different
tissues of buffalo calves (Bubalus bubalis). Onl J Vet Res.,
23 (10):977-988,
2019. Toll-like
receptors (TLR9) recognize unmethylated CpG dinucleotides in bacterial or viral DNA and trigger
innate immune response. Synthetic CpG oligodeoxynucleotides (ODN) mimic the effect of naturally
occurring CpG motifs in activation of mammalian
innate immune responses through TLR9.
The potential of synthetic CpG-ODN as
therapeutic agents and vaccine adjuvants has been demonstrated in animal models
of infectious diseases, allergy and cancer. Proper targeting of CpG ODN for the induction of innate immune defenses
requires an understanding of TLR9 expression by different immune compartments.
In the present study, transcriptional expression of TLR9 was studied in variety
of lymphoid tissues (mesenteric lymphnode, tonsil and
spleen), blood and mucosal tissues (lungs and intestine) of buffalo calves. The
abundance of TLR9 in different tissues was determined by normalizing the
integrated density values (IDV) of TLR9 expression by that of GAPDH, a
housekeeping gene. TLR9 mRNA expression levels were also determined and
validated by real-time PCR. All tissues and peripheral blood mononuclear cells
(PBMCs) exhibited variable mRNA expression of TLR9. The higher expression of
TLR9 was exhibited by lymphoid tissues as compared to PBMCs, lungs and the
intestine.
Key words: TLR9, mRNA expression, Buffalo, Real time PCR.
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