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OJVRTM

Online Journal of Veterinary Research

Volume 24(8): 475-482, 2020.


Survey of Brucella canis in dogs in a small animal hospital setting.

 

Sina sakhaii Far, Seyed Javid Aldavood, Fatemeh Gheitanchi, Sana Razavi Fard

 

Department of Small Animal Internal Medicine, Faculty of Veterinary Medicine, University of Tehran

 

ABSTRACT

 

Far SS, Aldavood SJ, Gheitanchi F, Fard SR., Survey of Brucella canis in dogs in a small animal hospital setting, Onl J Vet Res., 24(8): 475-482, 2020. Brucella canis causes brucellosis and reproductive failure in dogs but can also infect humans causing fever, chills, malaise, peripheral lymphadenomegaly, and splenomegaly. However, B. canis infection in dogs is underrecognized by serologic data, transmission patterns, and regulations in context of a zoonosis. Therefore development of appropriate diagnostic methods in dogs is necessary. Various methods have been developed to diagnose B.canis including serology and polymerase chain reaction (PCR). We tested 47 female and 73 male dog blood samples referred to the Small Animal Hospital of the Faculty of Veterinary Medicine, University of Tehran for presence of B canis. DNA from blood was extracted using DNA extraction kit and PCR test was carried out on the samples. A (825-bp of the gyrase) gene subunit-A was used in the PCR detection. We found no B.canis in any sample suggesting 0 prevalence in this case.

 

Keywords: Brucella canis, Brucellosis, Dog, polymerase chain reaction, electrophoresis.


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