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OJVRTM
Online Journal of Veterinary Research©
Volume 18(9):
743-749, 2014.
Serological
and molecular analysis of Brucella melitensis
and Brucella abortus in a human cohort (Iran)
Mohammad Kargar1*,
Farnaz Dehghani1, Abbas Dousti2,
Somayeh Handali3
1Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, 2Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, 3Nanotechnology
Research Center, Ahvaz Jundishapur University of
Medical Sciences, Ahvaz, Iran
ABSTRACT
Kargar M, Dehghani F, Dousti A, Handali S., Serological and molecular analysis of Brucella melitensis and Brucella abortus
in a human cohort (Iran), Onl J Vet Res., 18(9): 743-749, 2014. We report the prevalence of Brucella
amongst 1500 nomads from Iran using ELISA and PCR. Samples were screened by the
Rose-Bengal test and tittered with 2ME and Wrights test. Antibody titers
against Brucella IgG and IgM and Hemi-Nested PCR on Bru-F
Bru-R primers and Bm-F and
RR primers to B. abortus and B. melitensis respectively,
were determined. Ten percent or 156 samples were positive to Brucella spp., but no correlation was found between
positives with month of sampling, region, sex, or type of contiguity. Ninety three percent (145 samples) were
positive for IgG and 121 (77.56%) for IgM. PCR showed that of 49 (3.26%)
positive samples 79.59% were B. melitensis and
(6.12%) B. abortus. There was no significant correlation between
ELISA and PCR tests. The high prevalence
of Brucellosis among Nomads in Fars, Iran, was probably due to life style.
Key
words: Brucella melitensis. B. abortus, serological, Fars united.
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