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Online Journal of Veterinary Research

Volume 23 (10):956-960, 2019.  

Detection of infectious bronchitis 4/91 strain virus by RT-PCR in epididymis of Ross 308 roosters.


Nili Hassan (DVM, PhD)1, Hosseini Arsalan (DVM, PhD)2, Farinpour Amjad (DVM, PhD)3.


1Poultry Research Center, 2Pathobiology, School of Veterinary Medicine, Shiraz University, 3Animal Science, University of Kurdistan, Sanandaj, Iran.




Hassan N, Arsalan H, Amjad F., Detection of infectious bronchitis 4/91 strain virus by RT-PCR in epididymis of Ross 308 roosters., Onl J Vet Res., 23 (10):956-960, 2019. Avian infectious bronchitis (AIBV) was initially considered to be a disease primarily of the respiratory and female reproductive tract of chicken. There is little information concerning tropism of AIVB 4/91 virus in reproductive tract of roosters. One group of 45 Ross 308 chicks were inoculated 0.1ml intranasal attenuated live AIBV 4/91 vaccine at 2, 6, 10 and 14 weeks age. Controls (n=45) were inoculated 0.1 ml saline. Blood was taken at intervals for AIVB antibody titer, PCR and trachea, cecal tonsil, kidney and testes with or without epididymis for RNA extraction. All vaccinated roosters developed anti AIBV antibodies by 16 wks whereas unvaccinated controls remained negative throughout. We found no histological differences between testes or epididymis between controls and vaccinated birds at any age. By PCR we detected IAVB 4/91 in 42% cecal tonsils, 36% trachea, 25% kidney and 17% in epididymis. This study confirms that IBV 4/91 strain can infect the epididymis of roosters. We find that RT-PCR can be used to detect IBV 4/91 strain directly from different infected tissues.


Key-words:Infectious bronchitis, variant 4/91,Testicular tissue, Rooster, PCR.