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OJVRTM
Online Journal
of Veterinary Research©
Volume 23 (10):956-960, 2019.
Detection
of infectious bronchitis 4/91 strain virus by RT-PCR in epididymis of Ross 308
roosters.
Nili Hassan (DVM, PhD)1,
Hosseini Arsalan (DVM, PhD)2, Farinpour Amjad (DVM, PhD)3.
1Poultry Research Center, 2Pathobiology, School of
Veterinary Medicine, Shiraz University, 3Animal Science, University
of Kurdistan, Sanandaj, Iran.
ABSTRACT
Hassan
N, Arsalan H, Amjad F., Detection of infectious bronchitis 4/91 strain
virus by RT-PCR in epididymis of Ross 308 roosters., Onl
J Vet Res., 23 (10):956-960, 2019. Avian infectious bronchitis (AIBV) was
initially considered to be a disease primarily of the respiratory and female
reproductive tract of chicken. There is little information concerning tropism
of AIVB 4/91 virus in reproductive tract of roosters. One group of 45 Ross 308
chicks were inoculated 0.1ml intranasal attenuated live AIBV 4/91 vaccine at 2,
6, 10 and 14 weeks age. Controls (n=45) were inoculated 0.1 ml saline. Blood was taken at intervals for AIVB antibody
titer, PCR and trachea, cecal tonsil, kidney and
testes with or without epididymis for RNA extraction. All vaccinated roosters
developed anti AIBV antibodies by 16 wks whereas
unvaccinated controls remained negative throughout. We found no histological
differences between testes or epididymis between controls and vaccinated birds
at any age. By PCR we detected IAVB 4/91 in 42% cecal
tonsils, 36% trachea, 25% kidney and 17% in epididymis. This study
confirms that IBV 4/91 strain can infect the epididymis of roosters. We find
that RT-PCR can be used to detect IBV 4/91 strain directly from different
infected tissues.
Key-words:Infectious
bronchitis, variant 4/91,Testicular tissue, Rooster, PCR.