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OJVRTM

 

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume 28 (11): 797-813, 2024.

Infectious bronchitis vaccine 4/91 virus in blood and tissues of roosters.

 

Nili Hassan DVM PhD, Hosseini Arsalan DVM PhD, Farinpour Amjad DVM PhD.

 

School of Veterinary Medicine, Shiraz University, Iran.

 

ABSTRACT

 

Hassan N,  Arsalan H, Amjad F., Infectious bronchitis vaccine 4/91 virus in blood and tissues of roosters, Onl J Vet Res., 28 (11): 797-813, 2024.  Ross broilers (45) were inoculated 0.1ml intranasal attenuated live virus with 104.5 EID50 viral particles per dose type 4/91 at 2, 6, 10 and 14 weeks age.  Controls (45) were inoculated 0.1ml saline. Whole blood for RNA, cDNA, ELISA and antibody was taken days 35, 48, 63, 77, 91 and 105. For microscopy, 9 roosters from each group were sacrificed at 3, 7, 11 and 15 wk for tissues virus. Vaccinated birds developed antibodies to infectious bronchitis virus by ELISA by 16 weeks whereas controls were negative. Amplified cDNA from vaccinated birds from trachea, cecal tonsil, kidney, testis without or without epididymis were found at predicted 235bp whereas unvaccinated controls were negative. There were no microscopic differences in testes between vaccinated and controls but whole blood and testis without epididymis of infected chickens did not yield 235 bp product. The virus was found in trachea, cecal tonsils, kidneys and epididymis of experimentally infected chickens by RT-PCR of S1- gene oligonucleotide. Detection varied 42% for cecal tonsils to 17% for epididymis.

 

KEY-WORDS: Infectious bronchitis variant 4/91, live vaccine, antibody, tissues.

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