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OJVRTM
Online Journal of Veterinary Research©
Volume 17 (4): 159-166, 2013. Redacted 2017.


Detection of avian Metapneumovirus infection in broilers by nested RT-PCR

 

Hesami G1, Seyfi Abad Shapouri MR, DVM PhD,2 Mayahi M. DVM PhD3  

 

2Departments of Pathobiology and  Clinical Sciences, 1School of Veterinary Medicine, Shahid Chamran University, Ahwaz, Iran.

 

ABSTRACT

 

Hesami G, Seyfi Abad Shapouri MR, Mayahi M.,  Detection of avian Metapneumovirus infection in broilers by nested RT-PCR, Onl J Vet Res., 17 (4): 159-166, 2013.  Avian Metapneumovirus (aMPV) is the casual agent of Turkey Rhinotracheitis (TRT) which also causes a respiratory infection in chickens, resulting in Swollen Head Syndrome. aMPV infection in chicken flocks gives rise to considerable economic losses. aMPV infections were detected by RT-PCR in broiler flocks in Ahwaz, Iran. Fifty broiler flocks were sampled at slaughter via tracheal swabbing. RNA was extracted and RT-PCR based on viral N gene sequence specific primers (Nd and Nx) was performed. Positive results were confirmed by a semi-nested PCR.   aMPV was detected in 28% of samples. We report prevalence of aMPV infections and describe a novel semi nested PCR to confirm Nd/Nx in broilers.

 

Keywords: Avian Metapneumovirus, N gene, RT-PCR, Semi-nested PCR, Broiler flocks, Ahwaz


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