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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 25 (10):695-700, 2021.
Effect of branched chain amino acid supplement on hydrogen peroxide induced kidney and liver toxicity in Albino rabbits.
Fahad Saber Owain, Ahmed T Taha, Yaser AM Sulaiman.
Department of Animal Production, College of Agriculture, University of Tikrit, Iraq.
Owain FS, Taha AT, Sulaiman YA., Effect of branched chain amino acid supplement on hydrogen peroxide induced kidney and liver toxicity in Albino rabbits, Onl J Vet Res., (9):695-700, 2021. Blood was taken 12hrs after fasting at days 30 and 60 from marginal ear veins of 6 groups of 6 albino rabbits each given 0.02ml hydrogen peroxide and standard diet (H2O2) with or without 0.05, 0.1 or 0.2% branched chain amino acids (BCAA) or 0.1% leucine daily for 2 months. Six controls were given water and basal diet. Serum alanine and aspartate transaminases, urea and creatinine were determined by kit. Compared with controls, chronic H2O2 administration enhanced serum urea 40% by day 30 and 44% by day 60, whereas creatinine increased 70-77%. (P < 0.05) In rabbits supplemented branched amino acids (BCA) we still found increased urea but only 14-24% at day 30 and 7-15% by day 60. With BCA, creatinine increased 48-67%. We found no significant differences between 30 and 60 days data. However 60 day values were greater than day 30 probably due to prolonged H2O2 administration. We found that H2O2 boosted alanine transaminase 43-47% but with 0.1-0.2% BCA supplement, reduced levels back to normal by day 60. Changes in serum aspartate transaminase were similar. Our results suggest that BCA inhibited rises in urea and liver enzymes due to hydrogen peroxide induced toxicity in rabbits.
Key words: Branched amino acid, hydrogen peroxide, AST, ALT, Urea, Creatinine, rabbit.