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OJVRTM
Online Journal of
Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 28(5): 278-284, 2024.
PCR-plasmid vector for recombinant bovine herpesvirus
De Souza VF1,2, Dellagostin OA2, Roehe PM1,2.
1Centre of
Veterinary Research Desidério Finamor , Eldorado do Sul, RS, 2Centre of Biotechnology, Federal
University of Pelotas RS, Microbiology,
Virology Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, RS,
Brazil.
De
Souza VF, Dellagostin OA, Roehe PM., PCR-plasmid vector for recombinant bovine
herpesvirus, Onl J Vet Res., 28(5): 278-284, 2024. We report
cloned polymerase chain reaction (PCR) for plasmid recombinant bovine
herpesvirus type 5 (BHV-5). PCR was performed to amplify flanking sequence 5’
and 3’ regions of glycoprotein E (gE) gene of BHV-5 to yield gE-
vector for subcloning step amplification
of enhanced green flourescent protein (EGFP) reporter gene subcloned between 5’
and 3’ gE DNA fragments for gE-EGFP vector. This vector can be used
for production of recombinant BHV-5 to improve investigation of deleted viral
samples. The protocol generates plasmids for recombinant DNA viruses.
KEYWORDS:
PCR, Cloning, Plasmid, EGFP, BHV-5, Bovine herpesvirus.
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