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Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X


Volume 28(5): 278-284, 2024.

PCR-plasmid vector for recombinant bovine herpesvirus


De Souza VF1,2, Dellagostin OA2, Roehe PM1,2.


1Centre of  Veterinary Research Desidério Finamor , Eldorado do Sul, RS,  2Centre of Biotechnology, Federal University of Pelotas RS,  Microbiology, Virology Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.




De Souza VF, Dellagostin OA, Roehe PM., PCR-plasmid vector for recombinant bovine herpesvirus, Onl J Vet Res., 28(5): 278-284, 2024. We report cloned polymerase chain reaction (PCR) for plasmid recombinant bovine herpesvirus type 5 (BHV-5). PCR was performed to amplify flanking sequence 5’ and 3’ regions of glycoprotein E (gE) gene of BHV-5 to yield gE- vector for  subcloning step amplification of enhanced green flourescent protein (EGFP) reporter gene subcloned between 5’ and 3’ gE DNA fragments for gE-EGFP vector. This vector can be used for production of recombinant BHV-5 to improve investigation of deleted viral samples. The protocol generates plasmids for recombinant DNA viruses.


KEYWORDS: PCR, Cloning, Plasmid, EGFP, BHV-5, Bovine herpesvirus.