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Online Journal of Veterinary Research©
24(7): 438-448, 2020.
Bone marrow stem cell implant for articular cartilage defects in Guinea pigs.
Davood Mehrabani1,2,3,4, Mohammad Reza Sadraei5*, Nader Tanideh1, Mehrzad Lotfi6, Shahrokh Zare1, Mina Rabiee1
1Stem Cell and Transgenic Technology Research Center, 2Burn and Wound Healing Research Center, 3Comparative and Experimental Medicine Center, 6Imaging Research Center, Department of Radiology, Shiraz University of Medical Sciences, Shiraz, Iran. 4Li Ka Shing Center for Health Research and Innovation, University of Alberta, Edmonton, AB, Canada. 5Department of Biology, Science and Research Branch, Shiraz Islamic Azad University, Fars, Iran
Mehrabani D, Sadrae MR, Tanideh N, Lotfi M, Zare S, Rabiee M., Bone marrow stem cell implant for articular cartilage defects in Guinea pigs, Onl J Vet Res., 24(7): 438-448, 2020. Mesenchymal stem cells can differentiate into cartilage tissue. We describe effect of bone marrow-derived stem cell implant on full-thickness femoral articular cartilage defect in Guinea pigs. Under anesthesia, groups of 7 animals each were induced an articular defect and then implanted with stem cells. For sham guinea pigs, the anterior cruciate ligament was cut to induce osteoarthritis for 12 weeks but without cell implant. Controls were normal. Before and after cell therapy, femur-tibia joints were evaluated histologically and by radiography. Spindle shape stem cells exhibited plastic adherence, osteogenic and adipogenic differentiation with doubling time of 65.6h. RT-PCR revealed stem cell expression of CD44 and CD90 but absence of CD34 suggesting mesenchymal properties. Radiography at 12 weeks in shams revealed joint deformation, osteophytes, loss of joint space and knee osteoarthritis whereas in those implanted stem cells joint were healed. Similarly microscopy of joint tissue showed bone growth. Findings suggest that bone marrow stem cell implants may enhance knee cartilage repair in guinea pigs.
Key words: Bone marrow, Mesenchymal stem cells, Cartilage defect, Osteoarthritis, Healing, Guinea pig.