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OJVRTM
Online Journal of Veterinary
Research©
(Including
Medical and Laboratory Research)
Established
1994
ISSN
1328-925X
Volume 28(6): 334-340, 2024.
Effects of cryopreservation on caprine sperm.
Madurantakam SN, Masilamani JE.
Semen Bank, Department of Animal
Genetics & Breeding, Madras Veterinary College, Tamilnadu
Veterinary and Animal Sciences University Chennai, India.
Maduratakaman SN, Masilamani JE.,
Effects of cryopreservation on caprine sperm, Onl J
Vet Res 28(6): 334-340, 2024. Twenty seven semen samples were collected in artificial vagina from Boer grade bucks with 2 samples collected twice weekly for volume,
color, consistency, activity, concentration, motility, mortality and
abnormalities. Sperm was cryopreserved
in tris-egg yolk based dilutor with 7% glycerol.
After 4 hours equilibration at 5˚C, samples were packaged in 0.25 ml mini
straws frozen by exposure to liquid nitrogen vapor horizontally. The frozen
straws were plunged in liquid nitrogen for freezing at -196˚C.
Hamilton-Thorne integrated visual optical system was used to analyze samples.
Compared with pre-frozen samples, thawed sperm motility declined (P < 0.05) ~47-52%,
velocity ~16% whereas straightness and
linearity declined ~9%.
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