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OJVRTM
Online Journal of Veterinary Research©
Volume 8 : 42-47, 2004. Redacted
2018.
Increased tyrosine hydroxylase
immunoreactivity in urinary bladder of cats with interstitial cystitis.
Reche Aa, Buffington TCAb, Hagiwara MKa,
Daniel AGTa
aDepartment of Medical Clinics Faculdade de Medicina Veterinaria, Universidade de Sao
Paulo, Brazil b. Departmet of Veterinary Clinical
Sciences, College of Veterinary Medicine, Ohio State University, Columbus,
Ohio-USA. Author to whom correspondence should be sent: Archivaldo
Reche Junior Address: Av. Prof. Dr. Orlando Marques
de Paiva, 87 – Ci. Universitária
– 05508-900 – São Paulo – Brazil Phone and Fax # 55 11 3091-1287 e-mail: valdorec@usp.br Supported by NIH – NIDDK
grant DK 47538.
ABSTRACT
Reche Jr. A, Buffington TCA, Hagiwara MK, Daniel AGT, Increased
tyrosine hydroxylase mmunoreactivity in the urinary
bladder of cats with interstitial cystitis, Onl J Vet
Res., 8 : 42-47, 2004. We identify and quantify the
sympathetic nervous fibers in the urinary bladder of healthy cats (6), and those (6) with interstitial cystitis (IC), but
with no clinical signs for at least 6 months prior to the study. All cats were
euthanized and immediately perfused transcardially
with Krebs-Ringer and 4% paraformaldehyde solutions. Urinary bladders were
collected and frozen at -70C for analysis. Tissue slides were processed by immunohistochemical avidin-biotin
method for localization of neurons containing tyrosine hydroxylase(TH).
An increased number of sympathetic nervous fibers was
observed in the lamina propria and muscular layer of
the urinary bladder of cats with IC compared to controls. The increased number
of catecholaminergic fibers in the urinary bladder of
cats with IC might be related to the neurogenic inflammation of the urinary
bladder.
KEY WORDS:
interstitial cystitis, cats, tyrosine hydroxylase, urinary bladder.