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Online Journal of Veterinary Research©
Volume 24 (6):312-327, 2020.
Effect of selenium loaded Ribes nigrum nanoparticles on genetic markers in male rats with D-galactose induced toxicity.
Masar Jabbar Jary Al-Kurdy1 and Khalisa Khadim Khudair2
1Nursing, Technical Institute in Al-Diwaniyah, AL-Furat AL-Awsat Technical University, 2Department of Physiology, biochemistry and Pharmacology, College of Veterinary Medicine, University of Baghdad, Iraq.
Jary Al-Kurdy MJ, Khudair KK., Effect of selenium loaded Ribes nigrum nanoparticles on genetic markers of male rats with D- galactose induced toxicity, Onl J Vet Res., 24 (6):312-327, 2020. Authors report effect of selenium nanoparticles loaded with black currant (Ribes nigrum) extract on serum caspase3, chromosomes, mitotic index, liver tunel cells and apoptosis in Wistar male rats given 150mg/kg D-galactose IP daily for 56 days. We generated 18-50nm spherical crystallite nanoparticles identified by UV spectroscopy, electron microscopy and X–ray diffraction. Groups of 8 rats each were then injected 150mg/kg D-galactose IP dissolved in 0.9% saline daily for 8 weeks (Group 1), gavaged 1mg/kg nanoparticles (2) or given both (3). Controls were given saline IP and orally (4). At days 14 and 56, cardiac blood was taken for caspase-3 and femur bone excised for chromosomal aberration and mitotic indices, and livers for DNA fragmentation and apoptosis. Compared with controls, we found significant (P < 0.05) declines in serum caspase (~-10 to -17%), and chromosomal acentric aberrations (~-4 fold) in rats given nanoparticles with or without galactose. In rats given only galactose we found large increases in serum caspase-A (~18%) chromosomal abberations (~12 fold) and mitotic indices (~34%). We found no tunnel liver cells in rats given selenium loaded Ribes nigrum nanoparticles with or without D galactose but present throughtout in those given only D-galactose. Results suggest that selenium loaded Ribes nigrum nanoparticles nanoparticles may reduce D- galactose genotoxicity in rats and exert anti-oxidants effects.
Key Words: Selenium, nanoparticles, black current, apoptosis, liver.