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Online Journal of Veterinary Research©
Volume 23 (10):989-998, 2019.
Formation of Pasteurella multocida biofilm on bentonite.
Rajagopal R1*, Krishnan Nair G1 , Mini M2, Leo Joseph3
1,2Department of Veterinary Microbiology, College of Veterinary and Animal Sciences and 3Kerala Agricultural University Poultry Farm, College of Veterinary and Animal Sciences, Kerala, India.
Rajagopal R, Krishnan NG, Mini M, Joseph L., Formation of Pasteurella multocida biofilm on bentonite, Onl J Vet Res., 23 (10):989-999, 2019. Formation of Pasteurella multocida biofilm was assessed in tryptone soya broth supplemented with bentonite. The organism adhered to bentonite with an average peak count of 1.54 × 106 CFU g-1 of BC, 3 days after inoculation, whilst planktonic cells reached a peak count of 8.10 × 108 CFU mL-1, 1 day post inoculation. The biofilm colony on agar medium appeared discernably different from planktonic colonies, though it reversed on subsequent subcultures. Biofilm cells appeared extremely capsulated.
KEY WORDS : Biofilm, Pasteurella multocida, bentonite clay, capsular polysaccharide, Maneval staining, phenol sulphuric acid method.