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OJVRTM
Online Journal of Veterinary Research©
(Including Medical
and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 27 (1): 18-22, 2023.
Effect of chlorine and iodine on milk Escherichia
coli biofilm cells.
Mohammad Hosein Movassagh
Ghazani, Guity Karim, Ali R
Ahmadzadeh, Jalil Dolgharisharaf
Faculty of Veterinary Medicine, Islamic
Azad University, Shabestar, Iran
ABSTRACT
Ghazani MH, Karim G, Ahmadzadeh AR, Dolgharisharaf J., Effect
of chlorine and iodine on milk Escherichia
coli biofilm cells, Onl J Vet Res., 27 (1): 18-22, 2023. Bacterial biofilms
consist of cells attached to each other and surfaces that can transmit food
borne pathogens and spoilage. E.coli was grown in broth 2ml culture
added to milk incubated 28°C for 48h for biofilm cells. We counted biofilm
cells at 10 days. Effect of 10-100 ppm chlorine and 1-50ppm iodine on biofilm
cells over 0-25min was evaluated. We found that E.coli formed biofilm on
milk contact rubber with cell density of 9.10 ± 0.15 log cfu/cm2.
Exposure to 10 ppm chloride for 10 min reduced 1.47 log cells, whereas 20, 50
and 100ppm for 15min reduced cells 1.89, 1.24 and 1.61 log,
respectively. Exposure to 1 ppm iodine for 15 min reduced 0.88 log
cells whereas 10 and 50 ppm for 5 min only 0.79 and 1.23 log cells but 20 ppm
for 10 min 1.49 log. However, E.coli O111
planktonic cells suspended in PBS with 7 × 108 cells/ml were killed
by 5min in 100ppm chlorine or 50ppm of iodine.
Keywords: Biofilm, Escherichia coli, Sanitizers,
Hypochlorite, Iodophor, Rubber.
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