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Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X


Volume 27 (1): 18-22, 2023.

Effect of chlorine and iodine on milk Escherichia coli biofilm cells.


Mohammad Hosein Movassagh Ghazani, Guity Karim, Ali R Ahmadzadeh, Jalil Dolgharisharaf


Faculty of Veterinary Medicine, Islamic Azad University, Shabestar, Iran




Ghazani MH, Karim G, Ahmadzadeh AR, Dolgharisharaf J., Effect of chlorine and iodine on milk Escherichia coli biofilm cells, Onl J Vet Res., 27 (1): 18-22, 2023. Bacterial biofilms consist of cells attached to each other and surfaces that can transmit food borne pathogens and spoilage. E.coli was grown in broth 2ml culture added to milk incubated 28C for 48h for biofilm cells. We counted biofilm cells at 10 days. Effect of 10-100 ppm chlorine and 1-50ppm iodine on biofilm cells over 0-25min was evaluated. We found that E.coli formed biofilm on milk contact rubber with cell density of 9.10 0.15 log cfu/cm2. Exposure to 10 ppm chloride for 10 min reduced 1.47 log cells, whereas 20, 50 and 100ppm for 15min reduced cells 1.89, 1.24 and 1.61 log, respectively. Exposure to 1 ppm iodine for 15 min reduced 0.88 log cells whereas 10 and 50 ppm for 5 min only 0.79 and 1.23 log cells but 20 ppm for 10 min 1.49 log. However, E.coli O111 planktonic cells suspended in PBS with 7 108 cells/ml were killed by 5min in 100ppm chlorine or 50ppm of iodine.


Keywords: Biofilm, Escherichia coli, Sanitizers, Hypochlorite, Iodophor, Rubber.