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Online Journal of Veterinary Research

Volume 24(9): 558-567, 2020.

Virulence genes and biofilm in methicillin resistant Staphylococcus aureus in buffalo milk.


Mawlood Abbas Ali Al-Graibawi (PhD), Narges Radhi Luti (BVS), Malak Wissam Mohammed Jabar (BVS).


Zoonosis, College of Veterinary Medicine, University of Baghdad, Iraq




Al-Graibawi MAA, Luti NR, Mohammed Jabar MW., Virulence genes and biofilm in methicillin resistant Staphylococcus aureus in buffalo milk, Onl J Vet Res., 24(9): 558-567, 2020. Buffalo raw milk samples (n=50) from Al-Fudaliah, Iraq were screened for methicillin resistant Staphylococcus aureus (MRSA) by streaking, mannitol salt and blood agars, and sub-cultured on Congo red media for biofilm. Isolates were identified by colony morphology, Gram stain, biochemistry and for mecA and blaZ genes by PCR. Methicillin resistance by Staphylococcus aureus antimicrobial susceptibility was determined by disc diffusion and PCR. Staphylococci spp. was found in 18 (36%) milk samples, 12 Staphylococcus aureus and 6 S. epidermidis. The isolates were ~100% methicillin resistant, ~80% oxacillin and ~40% to erythromycin and tetracycline. blaZ and mecA genes and biofilm were detected in both isolates.


Keywords: biofilm, Staphylococcus aureus, MRSA, buffalo milk, mecA gene, blaZ gene.