2020-2032. All
Rights Reserved. Online Journal of Veterinary Research.
You may not store these pages in any form except for your own personal use. All
other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the
before mentioned must be gained in writing from the publisher. This
article is exclusively copyrighted in its entirety to OJVR publications. This
article may be copied once but may not be, reproduced or re-transmitted without
the express permission of the editors.
OJVRTM
Online Journal of
Veterinary Research©
Volume 24(9): 558-567, 2020.
Virulence
genes and biofilm in methicillin resistant Staphylococcus
aureus in buffalo milk.
Mawlood Abbas Ali Al-Graibawi
(PhD), Narges Radhi Luti (BVS), Malak
Wissam Mohammed Jabar (BVS).
Zoonosis,
College of Veterinary Medicine, University of Baghdad, Iraq
ABSTRACT
Al-Graibawi MAA, Luti NR, Mohammed Jabar MW., Virulence genes and biofilm in methicillin
resistant Staphylococcus aureus in buffalo
milk, Onl J Vet Res., 24(9): 558-567, 2020. Buffalo raw milk samples (n=50) from Al-Fudaliah, Iraq were screened for
methicillin resistant Staphylococcus aureus (MRSA) by streaking, mannitol
salt and blood agars, and sub-cultured on Congo red media for biofilm. Isolates were identified by colony
morphology, Gram stain, biochemistry and for mecA and blaZ genes by PCR. Methicillin
resistance by Staphylococcus aureus
antimicrobial susceptibility was determined by disc diffusion and PCR. Staphylococci spp. was found in 18 (36%) milk samples, 12 Staphylococcus aureus and 6 S. epidermidis. The isolates were ~100% methicillin resistant,
~80% oxacillin and ~40% to erythromycin and tetracycline.
blaZ and mecA genes and biofilm were detected in both
isolates.
Keywords: biofilm, Staphylococcus
aureus, MRSA, buffalo milk, mecA
gene, blaZ gene.