MAIN


1996-2017 All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing  from the publisher. This article is exclusively copyrighted in its entirety to OJVR. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking: To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page


 

OJVRTM

Online Journal of Veterinary Research©

Volume 21(3):126-134, 2017.


Effect of Baicalein on Acinetobacter baumannii biofilm formation in vitro and vivo.

 

Mohammad Mostafa Nazari1*, Pegah Peik Azadi2, Hamed Shirazi Beheshtiha3

 

Department(s) of 1Microbiology and Cell research, 3Clinical Science Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran, 2Faculty of Earth and Life Sciences,  VU University Amsterdam, Amsterdam, The Netherlands

 

ABSTRACT

 

Nazari MM, Azadi PP, Beheshtiha HS., Effect of Baicalein on Acinetobacter baumannii biofilm formation in vitro and vivo, Onl J Vet Res., 21(3):126-134, 2017. Bacterial biofilm consists of cells enclosed in extracellular polymeric compounds. Biofilm can exhibit antibiotic resistance. Effect of Baicalein on formation of biofilm by Acinetobacter baumannii ATCC 19606 is described. Biofilm formation, minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) of Baicalein were determined in vitro. A Sub-MIC dose of Baicalein was used to determine its effect on biofilm formation of A. baumannii and to reduce transcriptional changes. Transcriptional effects were confirmed by electron microscopy and ethidium bromide cell-membrane permeability assessment showing that Baicalein had a significant effect on transcriptome of A Baumannii. In Vivo, 4-week-BALB/c anaesthetized mice were implanted under skin with biofilm grown on Teflon catheters. By 24h, mice were given 6800µg/kg, ie:136µg/20gr Baicalein IV daily. Mice were euthanized 3 days later and catheter, homogenized skin, spleen and liver samples were used to determine a CFU count. Mice with the implanted catheter but not given Baicalein were used as controls. Results suggested great gene dysregulation for cell membrane biogenesis, motility, multidrug efflux pump and pilus assembly system. In-vivo mice studies showed that Baicalein had poor antibacterial activity requiring MIC >1163 µg/ml for inhibition. In vitro, MBEC 136µg/ml inhibited A. baumannii biofilm.

 

Keywords:   Acinetobacter baumannii; Baicalein; Biofilm formation.


MAIN

 

FULL-TEXT(SUBSCRIPTION) or order article