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OJVRTM
Online Journal of
Veterinary Research©
Volume 21(3):126-134,
2017.
Effect of Baicalein
on Acinetobacter baumannii
biofilm formation in vitro and vivo.
Mohammad
Mostafa Nazari1*, Pegah Peik Azadi2, Hamed Shirazi Beheshtiha3
Department(s)
of 1Microbiology and Cell research, 3Clinical Science Faculty
of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran, 2Faculty
of Earth and Life Sciences, VU
University Amsterdam, Amsterdam, The Netherlands
ABSTRACT
Nazari MM, Azadi PP, Beheshtiha
HS., Effect of Baicalein on Acinetobacter baumannii biofilm formation in vitro and vivo, Onl J Vet Res., 21(3):126-134, 2017. Bacterial biofilm consists
of cells enclosed in extracellular polymeric compounds. Biofilm can exhibit
antibiotic resistance. Effect of Baicalein on formation
of biofilm by Acinetobacter baumannii ATCC 19606 is described. Biofilm formation,
minimum inhibitory concentration (MIC) and minimum biofilm eradication
concentration (MBEC) of Baicalein were determined in vitro. A Sub-MIC dose of Baicalein was used to determine its effect on biofilm
formation of A. baumannii
and to reduce transcriptional changes. Transcriptional effects were confirmed
by electron microscopy and ethidium bromide cell-membrane permeability
assessment showing that Baicalein had a significant
effect on transcriptome of A Baumannii. In
Vivo, 4-week-BALB/c anaesthetized mice were implanted under skin with biofilm
grown on Teflon catheters. By 24h, mice were given 6800µg/kg, ie:136µg/20gr Baicalein IV daily. Mice
were euthanized 3 days later and catheter, homogenized skin, spleen and liver
samples were used to determine a CFU count. Mice with the implanted catheter but
not given Baicalein were used as controls. Results
suggested great gene dysregulation for cell membrane biogenesis, motility, multidrug efflux pump and pilus assembly system. In-vivo mice studies showed that Baicalein had poor antibacterial activity requiring MIC >1163
µg/ml for inhibition. In vitro, MBEC
136µg/ml inhibited A. baumannii
biofilm.
Keywords: Acinetobacter baumannii;
Baicalein; Biofilm formation.
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