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OJVRTM
Online Journal of
Veterinary Research©
Volume 23 (12):1212-1228, 2019.
Bioactive peptides in waste sheep and goat abbatoir
meat products.
Sailaja Pirati1,
Naveen Kumar Pirati2, Jayasimha Rayalu Daddam3*
1Department of Toxicology,
ITR Laboratories, Canada, 2Department of Inhalation Toxicology, ITR
Laboratories, Canada 3Department of Biotechnology, Sri Yuva Biotech PVT.LTD, India.
ABSTRACT
Pirati
S, Pirati NK, Daddam JR., Bioactive
peptides in waste sheep and goat abbatoir meat
products, Onl J Vet Res., Volume 23 (12):1212-1228, 2019. We isolated peptides from abbatoir
waste sheep and goat products by electrophoresis (SDS-PAGE), spectroscopy
(FT-IR), scanning electron microscopy (SEM) and chromatography (LC/MS). Antimicrobial
and antioxidant activity of the proteins and nutritional value were determined.
We then determined effects of goat peptide on pharmacokinetics of hypertension
drugs hydrochlorothiazide, nifedipine and valsartan in
rats. Ammonium sulfate fractionation yielded higher protein content and
antimicrobial activity. Free amino acid ranged 5.54–48.75 mg/g but much higher
in goat meat protein compared with sheep.
SDS-PAGE extracts had peptide at 5-12
kDa and protein bands at 72 kDa
by LC/MS. FT-IR revealed secondary protein structure mostly of α-helix. Sheep
protein mostly was large molecular weight but peptides had low Mw. By SEM we observed
flake-like structures of different sizes and goat protein appeared coarser. We
found
30 peptide goat protein with mw 1027.84–9916.32 Da maximum
charge +9, for mw 4947.51 Da and minimum +3, Mw 2757.38 Da. We isolated 9 sheep
peptides and 2 proteins with
mw 1040.35–3986.65 Da and protein 11,430.35–15,178.48 Da with maximum +8, Mw
3986.65 Da, minimum charge +4 for mw of 2775.4276 Da suggesting a relatively
large number of charged peptides. Goat
peptide scavenging reached 75.3-88.2% at 2 mg/mL, which we attribute to its high amino acid content. The peptides
inhibited Escherichia coli and Candida albicans.
Our findings suggest that goat peptide increased bioavailabilty
of nifepidine but had no effect on pharmacodynamics
of hydrochlorodiazide or valsartan.
Keywords: Meat, Biopeptide,
Antihypertensive drugs, Nutritional evaluation, SDS-PAGE, LC/MS.
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