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Online Journal of Veterinary Research©
Volume 23 (12):1212-1228, 2019.
Bioactive peptides in waste sheep and goat abbatoir meat products.
Sailaja Pirati1, Naveen Kumar Pirati2, Jayasimha Rayalu Daddam3*
1Department of Toxicology, ITR Laboratories, Canada, 2Department of Inhalation Toxicology, ITR Laboratories, Canada 3Department of Biotechnology, Sri Yuva Biotech PVT.LTD, India.
Pirati S, Pirati NK, Daddam JR., Bioactive peptides in waste sheep and goat abbatoir meat products, Onl J Vet Res., Volume 23 (12):1212-1228, 2019. We isolated peptides from abbatoir waste sheep and goat products by electrophoresis (SDS-PAGE), spectroscopy (FT-IR), scanning electron microscopy (SEM) and chromatography (LC/MS). Antimicrobial and antioxidant activity of the proteins and nutritional value were determined. We then determined effects of goat peptide on pharmacokinetics of hypertension drugs hydrochlorothiazide, nifedipine and valsartan in rats. Ammonium sulfate fractionation yielded higher protein content and antimicrobial activity. Free amino acid ranged 5.54–48.75 mg/g but much higher in goat meat protein compared with sheep. SDS-PAGE extracts had peptide at 5-12 kDa and protein bands at 72 kDa by LC/MS. FT-IR revealed secondary protein structure mostly of α-helix. Sheep protein mostly was large molecular weight but peptides had low Mw. By SEM we observed flake-like structures of different sizes and goat protein appeared coarser. We found 30 peptide goat protein with mw 1027.84–9916.32 Da maximum charge +9, for mw 4947.51 Da and minimum +3, Mw 2757.38 Da. We isolated 9 sheep peptides and 2 proteins with mw 1040.35–3986.65 Da and protein 11,430.35–15,178.48 Da with maximum +8, Mw 3986.65 Da, minimum charge +4 for mw of 2775.4276 Da suggesting a relatively large number of charged peptides. Goat peptide scavenging reached 75.3-88.2% at 2 mg/mL, which we attribute to its high amino acid content. The peptides inhibited Escherichia coli and Candida albicans. Our findings suggest that goat peptide increased bioavailabilty of nifepidine but had no effect on pharmacodynamics of hydrochlorodiazide or valsartan.
Keywords: Meat, Biopeptide, Antihypertensive drugs, Nutritional evaluation, SDS-PAGE, LC/MS.