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OJVRTM
Online Journal of
Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 27 (6): 27 (6): 337-352,
2023.
Mycobacterium avium antigen immune proline glutamic acid model for Johne’s disease
Rajib Deb, PP Goswami, NS Prasad
Gene Expression Laboratory,
Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar, UP, India
ABSTRACT
Deb R, Goswami PP, Prasad NS., Mycobacterium avium antigen immune proline glutamic acid mouse model for Johne’s disease, Onl J Vet Res., 27 (6): 27 (6): 337-352, 2023. Mycobacterium
avium paratuberculosisis (MAP) causes Johne’s cell mediated immunity disease.
Genome sequencing could expose proline glutamic acid (PPE) protein MAP for immunity genes. We report a bicistrionic 34.9 kDa PPE protein MAP with
cytokine gene encoding murine gamma
interferon gene (IFNγ) and a monocistrionic PPE
mammalian vector. These were transfected by
HeLa cell line for expression determined by Western
blot and immunofluorescent recombinant sera. We evaluated immunoreactivity of
mono-bicistrionic DNA in mice by delayed
hypersensitivity (DTH) LTT, nitric oxide (NO) and
ELISA. DTH was greater in Internal ribosome entry site (pIR)
PPE/IFN than pIR
PPE mice, similar to LTT and NO production
assay where pIR PPE/IFN mice showed higher value than
pIR PPE group. ELISA titer of pIR
PPE/IFN was less than that with PPE only. Our findings
revealed cell mediated immunity (CMI) response of
PPE protein of MAP with IFNγ boosting T cell based
immunity in mice.
Key Words PPE 34.9, FJ032182, IFNγ, Bicstrionic expression , HeLa celline
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