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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 27 (6): 27 (6): 337-352, 2023.
Mycobacterium avium antigen immune proline glutamic acid model for Johne’s disease
Rajib Deb, PP Goswami, NS Prasad
Gene Expression Laboratory, Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar, UP, India
Deb R, Goswami PP, Prasad NS., Mycobacterium avium antigen immune proline glutamic acid mouse model for Johne’s disease, Onl J Vet Res., 27 (6): 27 (6): 337-352, 2023. Mycobacterium avium paratuberculosisis (MAP) causes Johne’s cell mediated immunity disease. Genome sequencing could expose proline glutamic acid (PPE) protein MAP for immunity genes. We report a bicistrionic 34.9 kDa PPE protein MAP with cytokine gene encoding murine gamma interferon gene (IFNγ) and a monocistrionic PPE mammalian vector. These were transfected by HeLa cell line for expression determined by Western blot and immunofluorescent recombinant sera. We evaluated immunoreactivity of mono-bicistrionic DNA in mice by delayed hypersensitivity (DTH) LTT, nitric oxide (NO) and ELISA. DTH was greater in Internal ribosome entry site (pIR) PPE/IFN than pIR PPE mice, similar to LTT and NO production assay where pIR PPE/IFN mice showed higher value than pIR PPE group. ELISA titer of pIR PPE/IFN was less than that with PPE only. Our findings revealed cell mediated immunity (CMI) response of PPE protein of MAP with IFNγ boosting T cell based immunity in mice.
Key Words PPE 34.9, FJ032182, IFNγ, Bicstrionic expression , HeLa celline