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Online Journal of Veterinary Research


Volume 26 (9): 673-675, 2022.


Chromatographic spectrometry for β2-agonists in bovine hair.


Vita Di Stefanoa*, Rosa Pitonzob, Vita Giacconec, Angelina Alongic,

Andrea Macalusoc, Vincenzo Ferrantellic


aDepartment of Biological, Chemical and Pharmaceutical Science and Technology (STEBICEF), b CGA, UNINETLAB, University of Palermo,c Istituto Zooprofilattico Sperimentale della Sicilia, Palermo


Di Stefano V, Pitonzo R, Giaccone V , Alongi A, Macaluso A, Ferrantelli V., Chromatographic spectrometry for β2-agonists in bovine hair, Onl J Vet Res.,  26 (9): 673-675, 2022. A rapid, simple and efficient method was developed for simultaneous analysis of illegal residual β2-agonists in cattle hair. β2-agonists were quantified by ultra high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) operating in positive multiple-reaction monitoring mode (MRM). The method was validated as quantitative confirmatory method according to the EU Decision 2002/657/EC: instrumental linearity, specificity, precision, recovery, ruggedness, decision limit (CCα) and detection capability (CCβ) were evaluated. The recovery were greater than 90% and the method appeared suitable for the control of these β2-agonists in cattle hair samples, in the range of 4-12 ng/g. This method can be used in routine analysis for quantification and confirmation of illegal residues in livestock production to prevent foodstuff contamination.


Key Words: Chromatography, Spectrometry, β2-agonists, Bovine, hair.