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OJVRTM

Online Journal of Veterinary Research©

 (Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume 26 (1):86-92, 2022.

In vitro cultivation of Besnoitia caprae on epithelial monkey, canine and baby hamster kidney cell lines.

 

Namavari M1,*, Namazi F2, Oryan A2, Mansourian M1, Rahimian A1, Tahamtan Y1

 

1DVM, PhD. Razi Vaccine and Serum Research Institute, Shiraz, Iran, 2DVM, PhD. Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

 

ABSTRACT

 

Namavari M, Namazi F, Oryan A, Mansourian M, Rahimian A, Tahamtan Y In vitro cultivation of Besnoitia caprae on epithelial monkey canine and baby hamster kidney cell lines, Onl J Vet Res., 26 (1):86-92, 2022. Epithelial monkey (Vero), canine (MDCK) and baby hamster (BHK21) kidney cell lines were inoculated with tachyzoites of Besnoitia caprae from BALB-c mice. Bradyzoites were detached from tissue cysts of an infected goat for transition to tachyzoites in mice then transferred onto cell lines. Only BHK21 cells inoculated with tachyzoites from the peritoneal cavity of mice supported parasites with highest replication. Highest growth for tachyzoites occurred in BHK21 without any replication in MDCK or Vero cells. Mice and chicken embryonated eggs were inoculated with tachyzoites obtained from BHK21 cell culture. Mice given 1×105 tachyzoites died, whereas those given 5×104 from BHK21 survived 60 DPI with 2 mice exhibiting alopecia and skin lesions. Microscopy of skin revealed mild necrosis of epidermis and follicular adnexa with some mononuclear cell infiltration. Skin from mice and chicken embryonated eggs infected with tachyzoites of BC-Pars revealed a single PCR of 755 bp. We find that that B. caprae can be culture in vitro on BHK21 cells.

 

Key words: Besnoitia caprae; Cell culture; BALB/c mice; Chicken embryonated eggs.

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