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OJVRTM
Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 28(6): 341-357, 2024.
Early diagnosis of brucellosis in goats.
Abd El-Razik
KA, Desouky HM, Ahmed WM
Department of Animal
Reproduction AI National Research Center, Dokki,
Giza, Egypt
ABSTRACT
Abd
El-Razik KA, Desouky HM,
Ahmed WM, Early diagnosis of
brucellosis in goats. Onl J Vet Res., 28(6): 341-357, 2024. Baladi does were inoculated Brucella melitensis
biovar 3 at 40-45 days gestation and blood taken every
7 days. Inoculated does showed severe respiratory disorders and abortion by 3 –
5 weeks. Br. melitensis
was isolated from the spleen, and lymph nodes from all inoculated does. For antibody
we used ELISA,
plate antigen (BAPAT),
rose bengal (RBPT), rivanol (RVT),
complement (CFT) and agglutination (TAT) tests. ELISA, BAPAT, RBPT and RVT tests detected antibody
by 4 to 7 days but CFT and TAT by 14th day, peaking by 1st
week for BAPAT and RBPT, 3-4th for TAT and RVT and 5th week
for CFT and ELISA. Antibodies remained high 18 weeks by BAPAT and RBPT, 16 for
RVT, 13 by ELISA and 11 weeks for TAT and CFT. Compared with controls, in
inoculated animals, we found a ~7-10 fold boost in lymphocyte transformations.
By microscopy we observed
diffuse suppurative and necrotizing placentitis, and ulcerative endometritis with large
colonies of intra and extra cellular Brucella.
Findings suggest RBPT should be combined with ELISA while CFT and/or PCR as
confirmatory tests.
Key words: goats – Brucella melitensis - serology - PCR –
pathology.
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