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OJVRTM
Online
Journal of Veterinary Research©
Volume
20(8):507-513, 2016.
Susceptibility
of avian Trichomonas strains to metronidazole
Mohammad
Rajabloo1, Seyed Mostafa Razavi1,
Gholamreza Hatam2, Fatemeh
Nejati1
1Department of
Pathobiology, School of Veterinary Medicine, Shiraz University, 2Basic
Sciences and Infectious Diseases Research Center, Medical School, Shiraz
University of Medical Sciences, Shiraz, Iran
ABSTRACT
Rajabloo M, Razavi SM, Hatam
G, Nejati F., Susceptibility of avian Trichomonas
strains to metronidazole, Onl J Vet Res., 20(8):507-513, 2016. Genetic
variations are possible causes for different sensitivity patterns of protozoa
against antiprotozoal compounds. Eleven isolates of T. gallinae
identified based on ITS1/5.8S/ITS2 region and 18S rRNA
gene including T. gallinae, T. tenax and T. vaginalis strains were subjected to
In vitro inhibition by metronidazole
after 24h incubation under aerobic and anaerobic conditions. Minimum lethal concentration (MLC) was set at ≥50 µg/ml under
aerobic and ˃12.5 µg/ml under anaerobic condition.
MLCs of 8 T. tenax-strains
(Type II of ITS1/5.8S/ITS2 region and type I of 18S rRNA
gene) varied 1.56-6.25 µg/ml
and those of other T. tenax (Type III of ITS1/5.8S/ITS2 region and type X of 18S rRNA
gene)
was 1.56 µg/ml under aerobic condition. The MLCs of T. tenax-like strains were less than those of T.gallinae-like strains in both studies. Under aerobic
conditions MLC’s for T. gallinae-like isolates
MR28 and MR30 were 12.5ug/ml, and higher compared
with other T. tenax isolates (0.8 to 6.25 µg/ml). Similar results
were achieved
under anaerobic condition. Results suggest that T. gallinae-like strains
were more resistant to metronidazole than T.tenax-like
strains.
Keywords: Drug
susceptibility, Metronidazole, T.gallinae-like
strain, T. tenax-like strain.
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