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OJVRTM
Online Journal of Veterinary Research©
Volume 22 (1): 87-93, 2018.
Effect
of ferrous ascorbate on in vitro capacitation
of crossbred cattle bull spermatozoa
Amrit K Bansal and Gurmail
S Bilaspuri
Department
of Animal Breeding & Genetics. Guru Angad
Dev Veterinary & Animal Sciences University, Ludhiana, Punjab, India.
ABSTRACT
Bansal AK, Bilaspuri
GS., Effect of ferrous ascorbate on in vitro capacitation of crossbred cattle
bull spermatozoa, Onl J Vet Res., 22 (1): 87-93,
2018. Effect of mild lipid
peroxidation (LPO) in crossbred cattle bull spermatozoa induced by ferrous
ascorbate (FeAA – 150 µM FeSO4 :
750 µM ascorbic acid) on sperm capacitation and acrosome reaction is described.
Fresh spematozoa from crossbred cattle bulls were
suspended in TALP medium (pH 7.4) controls or with ferrous ascorbate (FeAA) 150 µM FeSO4 : 750µM
ascorbic acid. The fractions were incubated for 6h at 37°C and assessed for
morphology, activity, acrosome reaction and changes in LPO level during sperm
capacitation. Results suggested that spermatozoa morphology remained unaffected
and spermatozoa underwent normal process of capacitation and acrosome
reaction. Our results suggest that FeAA may have caused a slight increase in reactive oxygen
species, LPO and oxidative stress without affecting other sperm functions.
Keywords: FeAA, sperm
capacitation, acrosome reaction, LPO, % hyperactivity.