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Online Journal of Veterinary Research©
Volume 22 (1): 87-93, 2018.
Effect of ferrous ascorbate on in vitro capacitation of crossbred cattle bull spermatozoa
Amrit K Bansal and Gurmail S Bilaspuri
Department of Animal Breeding & Genetics. Guru Angad Dev Veterinary & Animal Sciences University, Ludhiana, Punjab, India.
Bansal AK, Bilaspuri GS., Effect of ferrous ascorbate on in vitro capacitation of crossbred cattle bull spermatozoa, Onl J Vet Res., 22 (1): 87-93, 2018. Effect of mild lipid peroxidation (LPO) in crossbred cattle bull spermatozoa induced by ferrous ascorbate (FeAA – 150 µM FeSO4 : 750 µM ascorbic acid) on sperm capacitation and acrosome reaction is described. Fresh spematozoa from crossbred cattle bulls were suspended in TALP medium (pH 7.4) controls or with ferrous ascorbate (FeAA) 150 µM FeSO4 : 750µM ascorbic acid. The fractions were incubated for 6h at 37°C and assessed for morphology, activity, acrosome reaction and changes in LPO level during sperm capacitation. Results suggested that spermatozoa morphology remained unaffected and spermatozoa underwent normal process of capacitation and acrosome reaction. Our results suggest that FeAA may have caused a slight increase in reactive oxygen species, LPO and oxidative stress without affecting other sperm functions.
Keywords: FeAA, sperm capacitation, acrosome reaction, LPO, % hyperactivity.