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Online Journal of Veterinary Research©
Volume 22 (5):356-361, 2018.
Effect of aryl alkyl amine-N-acetyl-transferase gene polymorphism on melatonin in non-seasonal ewes
Saad Akram Hatif1, Laith Sofian Younis2
1College of Veterinary Medicine, University of Baghdad, 2College of Veterinary Medicine, University of Tikrit, Iraq
Hatif SA, Younis LS., Effect of aryl alkyl amine-N-acetyl-transferase gene polymorphism on melatonin in non-seasonal ewes, Onl J Vet Res 22 (5):356-361, 2018. Jugular blood was taken from 20 Awasi ewes lambing out of season during October, November and December 2016 for amplification and sequencing of Ovis aries AA-NAT gene. We also collected 40 blood samples for plasma melatonin at 3:00 PM and 9:00 PM from 20 non-seasonal ewes which had had estrus during the previous Spring. Two primer amplified regions of the AA-NAT gene were compared with BLAST to confirm the gene and PCR-RFLP was used to detect polymorphisms. Melatonin in plasma was measured with an ELISA kit. Results showed a missense mutation in exon III A 1672 R that made a change in arginine to arginine C glycine and histidine to arginine & histidine and in C 1690 Y from alanine to valine and alanine. Two silent mutations (C 1676 T and A 1721 G) showed no change in amino acids. AG and GG Ovis aries AA-NAT genotypes appeared as single nucleotide polymorphisms (SNP) A 1665 R and A 1665 G. SNP A 1665 R may enhance AA-NAT, melatonin levels and reproduction. We found significant differences (P<0.05) in plasma melatonin levels between GG (31.034 ± 2.06 pg/ml) and AG (27.0715 ± 1.52 pg/ml) genotypes.
Key words: AA-NAT gene, Polymorphism, melatonin ewes.
Raw data files supplied as appendix tables at end of document.