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Online Journal of Veterinary Research ©
Volume 25 (3): 173-184, 2021.
Effect of ion beam irradiation and adenosine on cordycepin production by Cordyceps militaris fungus.
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Fukui 910-8507, Japan
Das SK*, Masuda M, Sakurai A, Sakakibara M., Effect of ion beam irradiation on cordycepin production by Cordyceps militaris fungus, Onl J Vet Res., 25 (3): 173-184, 2021. We boosted cordycepin production from a mutant Cordyeps militaris fungus by ion beam irradiation with adenosine. By proton beam, 30 classes of 8-azaadenine and 28 classes of 8-azaaguanine resistant mutants were obtained of which A63-7, A63-8, A81-2, A81-6, G63-8, G81-3, G82-4 were selected due to antibacterial production of cordycepin. G81-3 produced 6.84g/l cordycepin compared with controls 2.45 g/l or 2.79 times higher. We then added 6g/L adenosine in media yielding 8.57 g/l or 28.10% more than controls (6.69 g/l). Consumption of glucose was greatest with 4g/l adenosine and least with 10g/L extending culture time. We also added glycine/yeast extract ratios 40/60, 50/50, 60/40, 70/30, 80/20 and 90/10 or separately as 10, 20 and 30 weight % yielding similar increases in cordycepin. However 90/10 ratio yielded 6.80 g/l 12.40% more than controls (6.05 g/l). Ratio of 40/60 weight yeast extract increased glucose consumption and 30 reduced it extending culture time. Our results suggested that ion beam irradiation with adenosine boosted cordycepin production by cordycepin fungus.
Key words: Ion beam, Cordycepin, Cordyceps militaris, Mutant, Additive.