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OJVR TM
Online Journal of Veterinary Research ©
Volume 25 (3): 173-184, 2021.
Effect of ion beam
irradiation and adenosine on cordycepin production by
Cordyceps militaris
fungus.
Department of Applied Chemistry and Biotechnology, Graduate School
of Engineering, University of Fukui, Fukui 910-8507, Japan
ABSTRACT
Das SK*,
Masuda M, Sakurai A, Sakakibara M., Effect of ion beam irradiation on cordycepin
production by Cordyceps militaris fungus, Onl J Vet Res., 25 (3): 173-184, 2021. We boosted cordycepin
production from a mutant Cordyeps militaris fungus
by ion beam irradiation with adenosine. By proton beam, 30 classes of
8-azaadenine and 28 classes of 8-azaaguanine resistant mutants were obtained of
which A63-7, A63-8, A81-2, A81-6, G63-8, G81-3, G82-4 were selected due to
antibacterial production of cordycepin. G81-3 produced 6.84g/l cordycepin compared with controls 2.45 g/l or 2.79 times
higher. We then added 6g/L adenosine in media yielding 8.57 g/l or 28.10% more
than controls (6.69 g/l). Consumption of glucose was greatest with 4g/l adenosine
and least with 10g/L extending culture time. We also added glycine/yeast
extract ratios 40/60, 50/50, 60/40, 70/30, 80/20 and 90/10 or separately as 10,
20 and 30 weight % yielding similar increases in cordycepin.
However 90/10 ratio yielded 6.80 g/l 12.40% more than controls (6.05 g/l). Ratio
of 40/60 weight yeast extract increased glucose consumption and 30 reduced it
extending culture time. Our results suggested that ion beam irradiation with adenosine
boosted cordycepin production by cordycepin
fungus.
Key words: Ion beam, Cordycepin,
Cordyceps militaris,
Mutant, Additive.
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