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OJVR TM

Online Journal of Veterinary Research

Volume 25 (3): 173-184, 2021.


Effect of ion beam irradiation and adenosine on cordycepin production by Cordyceps militaris fungus.

Das SK, Masuda M, Sakurai A, Sakakibara M.

 

Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Fukui 910-8507, Japan

 

ABSTRACT

 

Das SK*, Masuda M, Sakurai A, Sakakibara M., Effect of ion beam irradiation on cordycepin production by Cordyceps militaris fungus, Onl J Vet Res., 25 (3): 173-184, 2021. We boosted cordycepin production from a mutant Cordyeps militaris fungus by ion beam irradiation with adenosine. By proton beam, 30 classes of 8-azaadenine and 28 classes of 8-azaaguanine resistant mutants were obtained of which A63-7, A63-8, A81-2, A81-6, G63-8, G81-3, G82-4 were selected due to antibacterial production of cordycepin. G81-3 produced 6.84g/l cordycepin compared with controls 2.45 g/l or 2.79 times higher. We then added 6g/L adenosine in media yielding 8.57 g/l or 28.10% more than controls (6.69 g/l). Consumption of glucose was greatest with 4g/l adenosine and least with 10g/L extending culture time. We also added glycine/yeast extract ratios 40/60, 50/50, 60/40, 70/30, 80/20 and 90/10 or separately as 10, 20 and 30 weight % yielding similar increases in cordycepin. However 90/10 ratio yielded 6.80 g/l 12.40% more than controls (6.05 g/l). Ratio of 40/60 weight yeast extract increased glucose consumption and 30 reduced it extending culture time. Our results suggested that ion beam irradiation with adenosine boosted cordycepin production by cordycepin fungus.

 

Key words: Ion beam, Cordycepin, Cordyceps militaris, Mutant, Additive.


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