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OJVRTM
Online Journal of Veterinary Research©
Volume 18(3): 213-230,
2014. Redacted 2017.
Modified double antibiotic synergy test.
Navid Ziaei-Darounkalaei1, Mehrdad Ameri2, Taghi
Zahraei-Salehi3, Omid Ziaei-Darounkalaei4, Tahereh Mohajer-Tabrizi5 and Lotfollah
Bornaei6
1Department
of Veterinary Medicine, Faculty of Agriculture, Islamic Azad University, Yasuj Branch, 2Department of Pathology, Amgen
Inc., USA- mameri@amgen.com,3Department of Microbiology, Faculty of
Veterinary Medicine, University of Tehran,4Department of Orthopedic
Surgery, Tehran University of Medical Sciences, 5Department of
General Surgery, Gorgan University of Medical
Sciences, Gorgan, 6Department of Animal
Science, Faculty of Agriculture, Islamic Azad University, Yasuj
Branch, Yasuj, Iran.
Ziaei-Darounkalaei N, Ameri M, Zahraei-Salehi T, Ziaei-Darounkalaei O, Mohajer-Tabrizi
T, Bornaei L., Modified double antibiotic synergy
test, Onl J Vet Res., 18(3): 213-230, 2014. Concurrent use of two or
more antimicrobials is common in patients with complicated infectious disease.
A double antibiotic synergy test for assessing
the extent of synergistic or antagonistic effects of antimicrobial drugs on the
growth inhibition of Staphylococcus aureus and Escherichia coli is described. Performance of the method was evaluated by
testing 8 repeats of synergistic and antagonistic combinations of penicillin
and gentamicin and erythromycin and clindamycin against Staphylococcus aureus and Escherichia coli compared with the
traditional disk approximation technique. Based on average growth inhibition
zones, combination of penicillin G and gentamicin showed greater inhibition
compared with each individual antibiotic whereas a combination of erythromycin
and clindamycin had less inhibition. Results suggest that the method identified
combined antibiotic activity more efficiently than the traditional disk method.
Keywords, Antibiotic Inhibition test.
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