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OJVRTM
Online
Journal of Veterinary Research©
(Including Medical and Laboratory
Research)
Established 1994
ISSN 1328-925X
Volume 22
(11):1048-1053, 2018.
Prevalence
of anaplasmosis in cattle in Iraq detected by
microscopy and PCR.
Tariq A. Hameed, May H. Kawan
Department of Parasitology, College of Veterinary
Medicine, University of Baghdad, Iraq
ABSTRACT
Hameed TA, Kawan MH., Prevalence of anaplasmosis in cattle in Iraq detected by microscopy and
PCR, Onl J Vet Res., 22
(11):1048-1053, 2018. Blood was taken over 6 months from 143 female and 37 male cattle aged <1, 1-3, and
>3 years with severe fever (>41C) pale mucus membranes, emaciation and
reduced milk production from farms located around Baghdad, Iraq. Jugular blood was tested for anaplasma by microscopy and PCR. One PCR primer was used
for detection of Anaplasma marginale
using a complete sequence 16s ribosome. By microscopy we found incidence of A. marginale of
19.4% and 30% by PCR. We found no effect of age, but in females we found
infection of 17.2% (31/143) compared with only 2.2% (4/37) in males (P<0.01). The highest (P< 0.05) incidence for A. marginale
occurred during
April 7.78% (14/25)
with significant differences (P<0.05) with other months. We found no
difference in incidence between localities. We surmise that the higher
incidence detected by PCR compared with microscopy, may be due to previous
non-detectable infection.
Key-Words: Anaplasmosis, cattle, microscopy, pcr,
age, gender, season, locality, Iraq.
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