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Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X


Volume 22 (11):1048-1053, 2018.

Prevalence of anaplasmosis in cattle in Iraq detected by microscopy and PCR.


Tariq A. Hameed, May H. Kawan


Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Iraq




Hameed TA, Kawan MH., Prevalence of anaplasmosis in cattle in Iraq detected by microscopy and PCR, Onl J Vet Res., 22 (11):1048-1053, 2018. Blood was taken over 6 months from 143 female and 37 male cattle aged <1, 1-3, and >3 years with severe fever (>41C) pale mucus membranes, emaciation and reduced milk production from farms located around Baghdad, Iraq. Jugular blood was tested for anaplasma by microscopy and PCR. One PCR primer was used for detection of Anaplasma marginale using a complete sequence 16s ribosome. By microscopy we found incidence of A. marginale of 19.4% and 30% by PCR. We found no effect of age, but in females we found infection of 17.2% (31/143) compared with only 2.2% (4/37) in males (P<0.01). The highest (P< 0.05) incidence for A. marginale occurred during April 7.78% (14/25) with significant differences (P<0.05) with other months. We found no difference in incidence between localities. We surmise that the higher incidence detected by PCR compared with microscopy, may be due to previous non-detectable infection.


Key-Words: Anaplasmosis, cattle, microscopy, pcr, age, gender, season, locality, Iraq.