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OJVRTM

 

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

 

Volume 29 (4): 182-188, 2025.

Alveolar phagocytosis of wild or gdhA type Pasteurella multocida B:2 in cattle.

 

Trang CTH, Zamri-Saad M, Noordin MM, Jasni S

 

Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Malaysia.

 

ABSTRACT

 

Trang CTH, Zamri-Saad M, Noordin MM, Jasni S, Alveolar phagocytosis of Pasteurella multocida B:2 in cattle, Onl J Vet Res., 29 (4): 182-188, 2025.  Two groups of 3 healthy calves  were each inoculated 5ml intra-tracheal solution with B:2 3.3´1010  wild type and 5.1×109 CFU/mL live gdhA derivative of Pasteurella multocida B2. Calves were monitored and sacrificed at 48 hours. Then 250 ml of warm sterile phosphate buffer saline pH 7.2 was injected into lungs to extract alveolar macrophages and neutrophils. There was significant (p<0.05) difference in phagocytosis by alveolar macrophages and neutrophils between calves inoculated with wild-type (45 + 4.1%) and gdhA derivative (57 + 3.4%) of P. multocida B:2. At necropsy we observed subcutaneous edema at jaws, neck and brisket. lungs, trachea, epiglottis were congested, edematous and lymph nodes congested with petechial hemorrhages. However these signs did not occur in calves inoculated with gdhA derivative of P. multocida B:2. By PCR, we were unable to re-isolate P. multocida in calves inoculated with live gdhA derivative suggesting that the mutated organism did not survive in host    

 

Key words: Pasteurella multocida B:2, hemorrhagic septicemia, phagocytosis, cattle.

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