1994-2018 All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors


Online Journal of Veterinary Research

 Volume 22 (1): 57-70, 2018.

DNA Sequence of alphatoxin gene in Clostridium perfringens types


Effat MM1, Abdallah YA2


1Microbiology and Immunology dept, National Research Centre, Giza, and 2Veterinary Serum and Vaccine Research Institute, Abbassia, Egypt.




Effat MM, Abdallah YA, DNA Sequence of alphatoxin gene in Clostridium perfringens types, Onl J Vet Res 22 (1) 57-70, 2018. Alpha toxin is produced by all types of Clostridium perfringens. The genes encoding alpha toxin from Clostridium perfringens [A (chicken strain), A (rabbit strain), B, C and D] were PCR amplified using specific primers and the PCR products were examined on 1.5% (w/v) agarose gel and found to be the same as published data. Restriction enzyme analysis using two sets of enzymes (one set known to have recognition sites; Hinf1, EcoRV and Mse1 and the other set known to lack recognition sites Hind III, Pst1 and BamH1) were carried out. The first set of enzymes revealed the same cut specific for alpha toxin gene. However, the second set of enzymes revealed no cut which is consistent to the published data. The PCR products of α toxin gene from the five types were separately sequenced and aligned with all published α toxin genes of Clostridium perfringens. Identities among all studied alpha toxin gene sequences and with the published ones were nearly 96-98%. There are no any significant differences among these nucleotide sequences. It is concluded that α toxin gene sequences among different types of Clostridium perfringens are similar and highly conserved.


KEYWORDS: Clostridium perfringens, alpha toxin, phospholipase C, DNA sequencing.