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OJVRTM
Online
Journal of Veterinary Research©
Volume 22 (1): 57-70, 2018.
DNA Sequence of alphatoxin gene in Clostridium perfringens types
Effat MM1, Abdallah YA2
1Microbiology and
Immunology dept, National Research Centre, Giza, and 2Veterinary
Serum and Vaccine Research Institute, Abbassia, Egypt.
ABSTRACT
Effat MM,
Abdallah YA, DNA Sequence of alphatoxin gene in Clostridium
perfringens types, Onl J Vet Res 22 (1) 57-70, 2018. Alpha toxin
is produced by all types of Clostridium perfringens. The genes encoding
alpha toxin from Clostridium perfringens [A (chicken strain), A (rabbit strain),
B, C and D] were PCR amplified using specific primers and the PCR products were
examined on 1.5% (w/v) agarose gel and found to be the same as published data.
Restriction enzyme analysis using two sets of enzymes (one set known to have
recognition sites; Hinf1, EcoRV and Mse1 and the
other set known to lack recognition sites Hind III, Pst1 and BamH1) were carried
out. The first set of enzymes revealed the same cut specific for alpha toxin
gene. However, the second set of enzymes revealed no cut which is consistent to
the published data. The PCR products of α toxin gene from the five types were
separately sequenced and aligned with all published α toxin genes of Clostridium
perfringens. Identities among all studied alpha toxin gene sequences and
with the published ones were nearly 96-98%. There are no any significant
differences among these nucleotide sequences. It is concluded that α toxin
gene sequences among different types of Clostridium perfringens are similar
and highly conserved.
KEYWORDS: Clostridium perfringens,
alpha toxin, phospholipase C, DNA sequencing.