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OJVRTM

Online Journal of Veterinary Research

 Volume 22 (1): 57-70, 2018.


DNA Sequence of alphatoxin gene in Clostridium perfringens types

 

Effat MM1, Abdallah YA2

 

1Microbiology and Immunology dept, National Research Centre, Giza, and 2Veterinary Serum and Vaccine Research Institute, Abbassia, Egypt.

 

ABSTRACT

 

Effat MM, Abdallah YA, DNA Sequence of alphatoxin gene in Clostridium perfringens types, Onl J Vet Res 22 (1) 57-70, 2018. Alpha toxin is produced by all types of Clostridium perfringens. The genes encoding alpha toxin from Clostridium perfringens [A (chicken strain), A (rabbit strain), B, C and D] were PCR amplified using specific primers and the PCR products were examined on 1.5% (w/v) agarose gel and found to be the same as published data. Restriction enzyme analysis using two sets of enzymes (one set known to have recognition sites; Hinf1, EcoRV and Mse1 and the other set known to lack recognition sites Hind III, Pst1 and BamH1) were carried out. The first set of enzymes revealed the same cut specific for alpha toxin gene. However, the second set of enzymes revealed no cut which is consistent to the published data. The PCR products of α toxin gene from the five types were separately sequenced and aligned with all published α toxin genes of Clostridium perfringens. Identities among all studied alpha toxin gene sequences and with the published ones were nearly 96-98%. There are no any significant differences among these nucleotide sequences. It is concluded that α toxin gene sequences among different types of Clostridium perfringens are similar and highly conserved.

 

KEYWORDS: Clostridium perfringens, alpha toxin, phospholipase C, DNA sequencing.


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