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OJVRTM
Online Journal of Veterinary Research©
Volume (9):904-908, 2019.
Effect of crude,
sonicated and P. aeruginosa liposaccharide antigens on immune markers in
female albino rats.
Hala SR AL-Taee, Ikram
AA Al-Samarraae.
Department of Microbiology, College
of Veterinary Medicine, University of Baghdad, Iraq.
ABSTRACT
AL-Taee HSR,
Samarraae IAA., Effect of crude, sonicated and P.
aeruginosa liposaccharide antigens, on immune markers in female albino rats (9):904-908, 2019. We report blood immune marker responses
to crude, sonicated and liposaccharide P.
aeruginosa antigens in rats; Four groups of 5 rats
each were injected 1ml crude (1), sonicated (2) or killed antigen (3)
subcutaneously. Controls were injected 1 ml PBS (pH 7.2) (4). After 14 days, rats were given booster doses
of same antigens and at day 28 a delayed type hypersensitivity skin test (DHS)
was performed. Blood was taken every 2 weeks for immunoglobulin (IgG)
interleukin (IL-2) and phagocytosis tests. Compared with controls (P < 0.05),
sonicated antigen induced 74% phagocytosis, LPS 70% and killed vaccine 43%. For DHL test,
sonicated 52-29% and LPS 38-28% between 24-72h whereas killed antigen had no effect on DHS at
48 or 72h. For IgG, sonicated 64-57%, LPS 40-35%, Killed 29-20% and IL-2 66-74%, LPS 90-60%
and killed 27-60% between days 30-58, respectively. Our results suggested that
the sonicated P aeruginosa antigens
induced greatest immune marker responses in rats.
Keywords: P. aeruginosa antigens, ELISA, IL2,
IgG, rats.
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