MAIN


1996-2018. All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking: To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page


OJVRTM

Online Journal of Veterinary Research

 

Volume (9):904-908, 2019.


Effect of crude, sonicated and P. aeruginosa liposaccharide antigens on immune markers in female albino rats. 

 

Hala SR AL-Taee, Ikram AA Al-Samarraae.

Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Iraq.

 

ABSTRACT

AL-Taee HSR, Samarraae IAA., Effect of crude, sonicated and P. aeruginosa liposaccharide antigens, on immune markers in female albino rats (9):904-908, 2019. We report blood immune marker responses to crude, sonicated and liposaccharide P. aeruginosa antigens in rats; Four groups of 5 rats each were injected 1ml crude (1), sonicated (2) or killed antigen (3) subcutaneously. Controls were injected 1 ml PBS (pH 7.2) (4). After 14 days, rats were given booster doses of same antigens and at day 28 a delayed type hypersensitivity skin test (DHS) was performed. Blood was taken every 2 weeks for immunoglobulin (IgG) interleukin (IL-2) and phagocytosis tests. Compared with controls (P < 0.05), sonicated antigen induced 74% phagocytosis, LPS 70% and killed vaccine 43%. For DHL test, sonicated 52-29% and LPS 38-28% between 24-72h whereas killed antigen had no effect on DHS at 48 or 72h. For IgG, sonicated 64-57%, LPS 40-35%, Killed 29-20% and IL-2 66-74%, LPS 90-60% and killed 27-60% between days 30-58, respectively. Our results suggested that the sonicated P aeruginosa antigens induced greatest immune marker responses in rats.

Keywords: P. aeruginosa antigens, ELISA, IL2, IgG, rats.


MAIN

 

FULL-TEXT (SUBSCRIBE OR PURCHASE TITLE)