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Online Journal of Veterinary Research©
Volume (9):904-908, 2019.
Effect of crude, sonicated and P. aeruginosa liposaccharide antigens on immune markers in female albino rats.
Hala SR AL-Taee, Ikram AA Al-Samarraae.
Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Iraq.
AL-Taee HSR, Samarraae IAA., Effect of crude, sonicated and P. aeruginosa liposaccharide antigens, on immune markers in female albino rats (9):904-908, 2019. We report blood immune marker responses to crude, sonicated and liposaccharide P. aeruginosa antigens in rats; Four groups of 5 rats each were injected 1ml crude (1), sonicated (2) or killed antigen (3) subcutaneously. Controls were injected 1 ml PBS (pH 7.2) (4). After 14 days, rats were given booster doses of same antigens and at day 28 a delayed type hypersensitivity skin test (DHS) was performed. Blood was taken every 2 weeks for immunoglobulin (IgG) interleukin (IL-2) and phagocytosis tests. Compared with controls (P < 0.05), sonicated antigen induced 74% phagocytosis, LPS 70% and killed vaccine 43%. For DHL test, sonicated 52-29% and LPS 38-28% between 24-72h whereas killed antigen had no effect on DHS at 48 or 72h. For IgG, sonicated 64-57%, LPS 40-35%, Killed 29-20% and IL-2 66-74%, LPS 90-60% and killed 27-60% between days 30-58, respectively. Our results suggested that the sonicated P aeruginosa antigens induced greatest immune marker responses in rats.
Keywords: P. aeruginosa antigens, ELISA, IL2, IgG, rats.