©2021-2032 All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under International copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editor.

OJVRTM

Online Journal of Veterinary Research©

 (Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X

 

Volume 25 (12):869-888, 2021.

  Functional analysis of cytochrome P450 genes in Aedes Aegypti.

 

Amir Hossein Asgari Safdar1*

 

1Department of Animal Sciences, Faculty of Agriculture and Natural Resources, Science  and Research Branch, Islamic Azad University, Tehran, IRAN.

 

ABSTRACT

 

Asgari Safdar AH., Functional analysis of cytochrome P450 genes in Aedes Aegypti, Onl J Vet Res., 25 (12):869-888, 2021. Author describes role of CYP6AA5, CYP6AL1, CYP9J32, and CYP4J16 in detoxification of permethrin, cypermethrin and deltamethrin by RNA interference (RNAi) insecticide bioassay in adults and larvae of Aedes aegypti. RNAi fed to larvae with chitosan/dsRNA nanoparticles reduced transcripts CYP6AA5 ~-39%, CYP6AL1 ~-46%, CYP9J32 ~-47%, CYP4J16A ~-44% and CYP4J16B ~-41%. This boosted larval mortality 41.2% to cypermethrin when CYP6AA5 was inhibited and 46.0% to permethrin if CYP9J32 suggesting detoxification. In adult mosquitoes, RNAi reduced CYP6AA5 78%, CYP6AL1 80% and CY4J16A transcripts 87% 96h after injection of corresponding double-stranded RNAs (dsRNAs). Expression of CYP9J32 was suppressed ~-46.5% by 24h whereas CYP4J16B ~-78.2% at 72h. Exposure of adults mosquitoes injected with CYP6AA5 dsRNA to cypermethrin for 24 h increased mortality 29.3%. Different slopes (P < 0.02) and intercept (P < 0.01) susceptibility to cypermethrin in mosquitoes injected CYP6AA5dsRNA or GFP dsRNA suggested detoxification. 3D binding revealed distances between heme iron and aromatic hydroxylation sites were 9.2 for permethrin, 9.4 cypermethrin and 7.2 Å for deltamethrin whereas for aliphatic hydroxylation sites were 5.3, 2.8 and 2.9 Å, respectively. CYP6AA5 may metabolize cypermethrin and deltamethrin by aliphatic hydroxylation with heme iron.

 

Keys words: Cytochrome P450, Aedes aegypti, detoxification, RNA interference, homology modeling.

MAIN

 

FULL-TEXT (SUBSCRIBE OR PURCHASE TITLE)