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Online Journal of Veterinary Research

Volume 24(8): 449-457, 2020.

Effect of virulent newcastle disease virus before and after vaccination on serum acute phase protein response in Cobb broilers.


Mostafa Ghaemmagham, Habibollah Dadras, Saeed Nazifi.


Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran




Ghaemmagham M, Dadras H, Nazifi S., Effect of virulent newcastle disease virus before and after vaccination on serum acute phase protein response in Cobb broilers, Onl J Vet Res., 24(8): 449-457, 2020. Cobb-500 broilers were given live vaccine virus before or after challenge with virulent Newcastle disease virus (NDV) to determine serum acute phase protein response. Four groups of 50 broilers each were given live vaccine at 22 days age (1), vaccinated at day 22 then challenged with NDV at day 23 of age (2), challenged with NDV at 23 days age then given vaccine at day 24 (3) or challenged only at day 23 (4). Controls were not challenged nor vaccinated (5). Blood was taken at 3, 6, 12, 18, 24, 48, 72h, 6 and 9 days after first inoculation for serum haptoglobin, serum amyloid A and ovotransferrin. Compared with controls, in birds challenged and vaccinated 24h later mean serum haptoglobolin did not change significantly but vaccinated 48 later increased ~40% suggesting that delayed vaccination after challenge may boost this protein. In those vaccinated first and then challenged 24h later, mean serum haptoglobulin increased ~27% but in those only challenged ~40%. Serum amyloid A increased ~33% in birds vaccinated first and ~57% in those challenged first vaccinated 2 days later. In those challenged first but vaccinated only 24h later SAA did not vary. We found large increases (~81%) in ovotransferrin (OVT) in birds challenged only with NDV but none in controls or those challenged first but vaccinated 24h later. Other treatments induced rises of ~50%. Our results suggested that viral challenge strongly enhances serum acute phase proteins whereas vaccination per se only induced small rises. Vaccination of the chicks after natural NDV infection may boost these proteins.

Keywords: Acute phase response, VG/GA vaccine virus, Newcastle disease virus.