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Online Journal of Veterinary Research©
Volume 22 (9):811-822, 2018.
Effect of Cutibacterium acnes cell free antigen (CFAg) immunization, levofloxacin
and cinnamon oil on Cutibacterium acnes infection in rabbits.
Ghaith S Tuaimah1, Ahmed Q Al-Awadi2, Mais E Ahmed.3
1Department(s) of Pathology, College(s) of Veterinary Medicine, University of Kufa, Alkut 2University of Baghdad, 3Biology, College of Science, University of Baghdad, Aljihad, Iraq.
Tuaimah GS, AQ Al-Awadi, Ahmed ME., Effect of Cutibacterium acnes cell free antigen (CFAg) immunization, levofloxacin and cinnamon oil on Cutibacterium acnes infection in rabbits, Onl J Vet Res., 22 (9):811-823, 2018. C. acnes causes acne vulgaris most commonly in 12-24 year olds. Five albino rabbits were immunized at day 1 and 14 with Cutibacterium acnes cell free antigen (CFAg) and then infected intradermally with 0.1 ml C. acnes suspension (2×108 cfu/ml) at day 30. Two groups of five infected rabbits were then treated at Day 30 with 0.5% levofloxacin or 0.2% cinnamon oil twice daily for 12 days. Five control rabbits were infected intradermally with 0.1 ml 2×108 cfu/ml Cutibacterium acnes n (2×108 cfu/ml). At day 42, skin test (mm), serum IgG and IFN-γ were determined. In vitro, we found C. acnes minimum inhibitory concentrations for cinnamon oil 2mg/ml and levofloxacin 1.5 µg/ml, but resistant to clindamycin, azithromycin, erythromycin and metronidazole. Compared with controls, immunized rabbits showed significant increases in skin thickness (P < 0.01), serum IgG (P < 0.05) and IFN-γ levels (P < 0.01) and less pathology. In rabbits treated with cinnamon oil or levoflaxin we observed least pathology compared with other treatments. Results suggest that immunization with C. acnes CFAgs enhanced immunity and reduced lesions, and levoflaxin and cinnamon oil, may control acne.
Keywords: Cutibacterium acnes, cell free antigen (CFAg), cinnamon oil, levofloxacin, rabbit