2921-2032. All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking: To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page


Online Journal of Veterinary Research

Volume 25 (3): 238-244, 2021

Production and applications for acellular fish skin (Cyprinus carpio) scaffold.


Ali Saleh Mahdi1, Ahmed H. F. AL-Bayati2


1Department(s) of Surgery and Obstetrics, College(s) of Veterinary Medicine, 1Al- Qasim Green University, 2Baghdad University, Iraq





Mahdi AS, AL-Bayati AHF., Production and applications for acellular fish skin (Cyprinus carpio) scaffold, Onl J Vet Res., 25 (3): 238-244, 2021. We describe manufacture and bio-compatibility of a 3D acellular fish skin matrix sheet for tissue engineering applications. De-cellularization was confirmed by absence of cells and nuclei by eosin-hematoxilin stain. Scan electronic microscopy revealed a scaffold matrix of highly porous spongy structure with 155.17-300.63μm particles between pores of 40.83-120.08μm. Spectroscopy showed broad peaks with relatively low intensity at 2θ = 7.3, 24.7 and 33.2 due to semi-crystalline or amorphous organic JCPDS# 09-0432 collagen, proteoglycan and hyaluronic acid, JCPDS#035-0812 collagen, struvite and hydroxyapatite and PDF#50-2241 omega-3. This highly porous scaffold with ideal pore size could have tissue engineering applications which should be confirmed in vivo.


Keywords: (Cyprinus carpio), acellular skin matrix, sodium dodecyl sulfate.