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Online Journal of Veterinary Research

Established 1994

ISSN 1328-925X


Volume 22 (10):919-927, 2018.

Histomorphological effects of Metformin, Pioglitazone, Repaglinide and Acarbose on polycystic ovaries in NMRI mice.


Noori Fariba1, Parto Paria*2, Azadbakht Mehri1 , Bazaz Zohreh3 and Darvishnia Hamid4


1Department(s) of Biology., Faculty of Science, Razi University, Kermanshah, Iran, 2Biological Sciences, Prince Georges Community College, Largo, Maryland, USA, 3GMP inspector IFDA, 4Health Ministry Tehran, 4Biology, Payame Noor University, Tehran, Iran. *Corresponding author: partopx@pgcc.edu




Fariba N, Paria P, Mehr A, Zohreh B, Hamid D., Histomorphological effects of Metformin, Pioglitazone, Repaglinide and Acarbose on polycystic ovaries in NMRI mice, Onl J Vet Res., 22 (10):919-927, 2018. Polycystic ovaries (PCO) were induced by injection of testosterone enanthate (TE) in 5 groups of 5 immature NMRI female mice each for 4 weeks then treated with Metformin, Pioglitazone, Repaglinide and Acarbose for 2-4 weeks. Five of these mice were testosterone PCO controls. Body and ovary/body weights were measured and microscopy of ovaries was evaluated. In PCO controls, we found numerous cystic follicles but reduced follicle growth. Compared with PCO controls, in those given metformin, body weights declined 17% ovary/body ratio weight 36% and ovarian diameter 38% (p<0.05). Pioglitazone induced similar effects (P < 0.05). In mice given Metformin, Pioglitazone and Repaglinide we found less oocytes, pyknosis and vascularization and fewer primordial, primary, antral, cystic and atretic follicles but more pre-antral follicles (p < 0.05). With all treatments we found less polycystic ovaries, degenerated oocytes, pycnosis and vascularization. Results suggest that Metformin and Pioglitazone best ameliorated polycystic ovary syndrome in NMRI mice.


Keywords: Polycystic ovary syndrome, Metformin, Pioglitazone, Acarbose, Ripaglinide, MMRI mice.