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Online Journal of Veterinary Research©
Volume 25 (1): 61-69, 2021
Molecular identification and phylogenetic analysis of Chlamydia abortus isolated from ewes and goats in Iran
Fariba Taheri1, Abdulghaffar Ownag2, Karim Mardani3
1, 2Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, West Azarbaijan, Iran. 3Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Melbourne University, Melbourne, Australia.
Taheri F, Ownag A, Mardani K., Molecular identification and phylogenetic analysis of Chlamydia abortus isolated from ewes and goats in Iran, Onl J Vet Res., 25(1): 61-69, 2021. Chlamydia abortus is a major cause of failed pregnancy in sheep and goats. However, identification and phylogenetics of C. abortus strains in milk in sheep and goat in West Azerbaijan, Iran, remains unknown. We collected 180 sheep and 180 goat milk samples for nPCR 16S rRNA target gene of Chlamydia spp. The outer membrane protein gene (omp) was used for Nested-PCR and we found 8.6% C. abortus prevalence, 11.7 in sheep and 5.6% in goat milk (P < 0.05) with 1.5 in Southern, 5.7 Northern and 15.7% (P < 0.05) in Central Azerbaijan. We found 14.4% (P < 0.05) prevalence in animals with abortion history but only 4% in those without. In animals aged 0-2 years, prevalence was 2.8; 2-4 years 10.4 and >4 years, 17.2% (P < 0.05). Sequencing LN554883-CP024084-KP719159 revealed similarity to Gene bank in the same cluster. Of 31 milk samples positive to 16S-rRNA gene test, only 8 (P 0.05) were C. abortus by PCR of 479bp fragment of ompA gene. We found neither recombination, plasmid nor significant phylogenetic changes within our C. abortus. We conclude that sheep and goats are significant reservoirs for C. abortus, consumption of goat milk may be a higher risk factor, and that our Nested PCR readily detected chlamydia in milk.
Keywords: Chlamydia abortus, ewe, goat, milk, omp gene, Nested-PCR, sequencing.