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OJVRTM

 

Online Journal of Veterinary Research

 

 Volume 22 (5):417-424, 2018.


Prevalence of Toxoplasma gondii in abortive women in Iraq detected by ELISA and PCR.

 

Wafaa Ayad Al-Nuaimya and Tareq Jafaar Al-Jandeelb.

 

aCollege of Science, bCollege of Veterinary Medicine, Al-Muthanna University, Al-Muthana, Iraq.

 

ABSTRACT

 

Al-Nuaimy WA, Al-Jandeel TJ., Prevalence of Toxoplasma gondii in abortive women in Iraq detected by ELISA and PC., Onl J Vet Res., (5):417-424, 2018. Toxoplasmosis during pregnancy is a significant risk factor for adverse pregnancy outcomes in humans. One hundred twenty women from Al Muthanna, Iraq who had had a single or repeat abortion were tested. ELISA produced sensitivity of 75% and specificity 96.8% and RT-PCR produced similar results. By ELISA IgG incidence was 17.5 % and by RT-PCR 20%. Most early miscarriages occurred during the 1st trimester representing 50% of cases, 36.7% during the 2nd trimester and 13.3% during the 3rd trimester in stillbirth. We found 58(48.3%) with single miscarriage, and 62(51.7%) with 2 or 3 or more recurrent miscarriages. T. gondii detected by qtPCR was associated (P < 0.02) with miscarriages in the 1st trimester of pregnancy but by ELISA we found no significant differences between trimesters. We find a high prevalence for T. gondii in abortive women in Iraq probably due to multiple transmission modes inducing early and recurrent miscarriages.

 

Keywords : T.gondii, early miscarriages, Late miscarriages, Gestational age, qPCR.


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