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OJVRTM
Online Journal of
Veterinary Research©
Volume 19(4):
237-246, 2015.
Antibody response effect of fusing IgY and C3d gene segments to hemagglutinin9
(H9) gene on influenza DNA vaccines in chickens
Ghaemi Bafghi M. (DVM)1,
Bassami M.R. (DVM, PhD)2*, Hashemi Tabar Gh.R. (DVM, PhD)3, Saberi
M.R. (Pharm. D, PhD)4,
Haghparast A.R. (DVM, PhD)5, Dehghani H. (DVM, PhD)6
Division(s) of 1Biotechnology,
2Clinical Sciences 3,5Pathobiology 6Basic Sciences,
Faculty of Veterinary Medicine Ferdowsi University, 4School of Pharmacy,
Mashhad University of Medical sciences, Mashhad, Iran,
ABSTRACT
Bafghi M, Bassami MR, Tabar H, Saberi MR, Haghparast AR, Dehghani H., Antibody response effect of fusing IgY and C3d gene segments to Hemagglutinin9 (H9) gene on
influenza DNA vaccines in chickens, Onl J Vet Res., 19(4): 237-246, 2015. Hemagglutinin9
gene (PCAG-sH9) alone, fused to 4 or 8 repeats of 29 amino acids (P29) from C3d
coding sequence (PCAG-sH9+4/8P29C3d), fused to Fc part of IgY
coding sequence (PCAG-sH9+FcIgY) or fused to 8P29C3d and FcIgY
coding sequence (PCAG-sH9+8P29C3d+FcIgY) DNA vaccines were tested in Leghorn chickens.
Expression of DNA vaccine constructs were confirmed by
culture media and transfected cells with DNA vaccine plasmids by ELISA and
immunofluorescence assays. The vaccines were injected intramuscularly to H9
free groups of 9 chickens each at 8 and 11 weeks of age. Anti-H9 antibody was
measured by Hemagglutination Inhibition (HI) assay. Results
suggested that fusing Fc IgY gene to PCAG-sH9 did not
enhance immunogenicity of H9 DNA vaccine. In contrast, when 8P29C3d was fused
and PCAG-sH9+8 P29C3d+ Fc IgY
DNA vaccine was injected, immunogenicity increased significantly (P <0.0001).
This may have been due to a synergistic effect of 8 P29C3d and Fc IgY on PCAG-sH9.
Keywords:
C3d,
DNA vaccine, H9, IgY,
Influenza virus
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